Exogenously Applied Nitric Oxide Enhances the Drought Tolerance in Fine Grain Aromatic Rice (Oryza sativa L.)
Exogenously Applied Nitric Oxide Enhances the Drought Tolerance in Fine Grain Aromatic Rice (Oryza sativa L.)
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DOI：
10.1111/j.1439-037x.2009.00367.x
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发表时间：
2009-08-01
影响因子：
3.5
通讯作者：
Rehman, H.
中科院分区：
文献类型：
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作者：
Farooq, M.;Basra, S. M. A.;Rehman, H.
干旱胁迫是水稻可持续生产的一个严重威胁，它引起氧化损伤，扰乱植物水分关系，而外源施用一氧化氮（NO）可能有缓解这些影响的潜力。本研究探讨了NO在提高细粒香稻抗旱性中的作用。Basmati 2000)被评估。硝普钠作为NO供体，分别以50、100和150 μ mol l（-1）的剂量灌种和叶面喷施。将种子在相应溶液的曝气NO溶液中浸泡48 h，晾干后恢复原状。在温室内，将处理过的种子和未处理过的种子种在塑料罐中，并进行正常灌溉。在四叶期，除对照植株保持满负荷生长外，其余植株均受到干旱胁迫。通过在需要时浇水，旱情维持在田间能力的50%。维持两组对照；在干旱条件下和丰水条件下，两种水稻均不进行叶面施用或种子处理。干旱胁迫严重影响水稻生长，但两种施NO方式均能缓解干旱胁迫效应。水稻的抗旱性与维持组织水势、增强抗氧化能力、改善细胞膜稳定性和增强光合能力密切相关，这可能与NO的信号作用有关。叶面处理比种子处理更有效。在NO处理中，100 μ mol l（-1）叶面喷施效果更好。
Drought stress is a severe threat to the sustainable rice production, which causes oxidative damage and disturbs plant water relations, while exogenously applied nitric oxide (NO) may have the potential to alleviate these effects in rice plants. In this study, the role of NO to improve drought tolerance in fine grain aromatic rice (Oryza sativa L. cv. Basmati 2000) was evaluated. Sodium nitroprusside, a NO donor, was used at 50, 100 and 150 mu mol l(-1) both as seed priming and foliar spray. To prime, the seeds were soaked in aerated NO solution of respective solution for 48 h and dried back to original weight. Primed and non-primed seeds were sown in plastic pots with normal irrigation in a greenhouse. At four leaf stage, plants were subjected to drought stress except the controls, which were kept at full field capacity. Drought was maintained at 50 % of field capacity by watering when needed. Two controls were maintained; both receiving no NO treatments as foliar application or seed treatment, one under drought conditions and the other under well-watered conditions. Drought stress seriously reduced the rice growth, but both methods of NO application alleviated the stress effects. Drought tolerance in rice was strongly related to the maintenance of tissue water potential and enhanced capacity of antioxidants, improved stability of cellular membranes and enhanced photosynthetic capacity, plausibly by signalling action of NO. Foliar treatments proved more effective than the seed treatments. Among NO treatment, 100 mu mol l(-1) foliar spray was more effective.