copia-like retrotransposons are ubiquitous among plants.
copia-like retrotransposons are ubiquitous among plants.
复制标题
DOI：
10.1073/pnas.89.15.7124
复制
发表时间：
1992-08
影响因子：
11.1
通讯作者：
D. Voytas;M. P. Cummings;A. Konieczny;F. Ausubel;S. Rodermel
中科院分区：
文献类型：
--
作者：
D. Voytas;M. P. Cummings;A. Konieczny;F. Ausubel;S. Rodermel
转座遗传元件被认为是所有真核生物基因组的一个特征。然而，它们的鉴定在很大程度上是偶然的，主要限于受分子或基因审查的生物体。我们利用聚合酶链反应检测复制样元件逆转录酶序列，评估了复制样逆转录转座子（一类通过逆转录增殖的转座子）的系统发育分布。在64种植物和光合原生生物Volvox carteri中发现了复制样反转录转座子。植物种类包括苔藓植物、石松类、蕨类、裸子植物和被子植物等10个植物分类中的9个。对29个克隆PCR产物和一个玉米反转录转座子cDNA的DNA序列分析证实了这些序列是复制样逆转录酶序列，从而证明这类反转录转座子是植物基因组中普遍存在的成分。
Transposable genetic elements are assumed to be a feature of all eukaryotic genomes. Their identification, however, has largely been haphazard, limited principally to organisms subjected to molecular or genetic scrutiny. We assessed the phylogenetic distribution of copia-like retrotransposons, a class of transposable element that proliferates by reverse transcription, using a polymerase chain reaction assay designed to detect copia-like element reverse transcriptase sequences. copia-like retrotransposons were identified in 64 plant species as well as the photosynthetic protist Volvox carteri. The plant species included representatives from 9 of 10 plant divisions, including bryophytes, lycopods, ferns, gymnosperms, and angiosperms. DNA sequence analysis of 29 cloned PCR products and of a maize retrotransposon cDNA confirmed the identity of these sequences as copia-like reverse transcriptase sequences, thereby demonstrating that this class of retrotransposons is a ubiquitous component of plant genomes.