Basic fibroblast growth factor synthesis by human peritoneal mesothelial cells - Induction by interleukin-1
Basic fibroblast growth factor synthesis by human peritoneal mesothelial cells - Induction by interleukin-1
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DOI：
10.1016/s0002-9440(10)65516-2
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发表时间：
1999-12-01
影响因子：
6
通讯作者：
Offner, FA
中科院分区：
文献类型：
--
作者：
Cronauer, MV;Stadlmann, S;Offner, FA
腹膜间皮细胞是调节腹膜腔内细胞活动的独特位置，是各种细胞因子和生长因子的重要来源。本研究旨在分析人腹膜间皮细胞（HPMCs）合成和释放碱性成纤维细胞生长因子（bFGF）的能力，并表征其受炎症因子的调节。经特异性免疫测定，hpmc合成并释放大量bFGF。近80%的bFGF （1547 +/- 173 pg/10(5)个细胞）定位于细胞内，约20%的bFGF （357 +/- 27 pg/10(5)个细胞）与HPMC表面的细胞外基质成分相关。少量bFGF (
Peritoneal mesothelial cells are uniquely located to regulate cellular events in the peritoneal cavity and are an important source for various cytokines and growth factors. This study was conducted to analyze the capacity of human peritoneal mesothelial cells (HPMCs) to synthesize and release basic fibroblast growth factor (bFGF) and to characterize its regulation by inflammatory cytokines. HPMCs constitutively synthesized and released considerable amounts of bFGF as detected by a specific immunoassay. Almost 80% of bFGF (1547 +/- 173 pg/10(5) cells) was localized intracellularly, Approximately 20% of the bFGF (357 +/- 27 pg/10(5) cells) was associated with extracellular matrix components on the HPMC surface. Small amounts of bFGF (