Cell-specific adaptation of two flaviviruses following serial passage in mosquito cell culture
Cell-specific adaptation of two flaviviruses following serial passage in mosquito cell culture
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DOI：
10.1016/j.virol.2006.08.005
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发表时间：
2007-01-20
期刊：
影响因子：
3.7
通讯作者：
Kramer, Laura D.
中科院分区：
文献类型：
--
作者：
Ciota, Alexander T.;Lovelace, Amy O.;Kramer, Laura D.
西尼罗河病毒（WNV）是一种蚊媒黄病毒，于1999年在纽约市地区传入美国。尽管它在一个幼稚的环境中成功地建立和迅速传播，西尼罗河病毒已经经历了有限的演变，因为它的介绍。这种进化稳定性归因于为允许脊椎动物宿主和节肢动物载体中病毒复制的交替循环而做出的妥协。西尼罗河病毒的近亲圣路易斯脑炎病毒（SLEV）在美国定期爆发，其特征也是随着时间的推移发生有限的遗传变化。我们测量了在蚊子细胞培养物中连续传代的WNV和SLEV的表型和基因型变化，以澄清单个宿主细胞类型在黄病毒适应和进化中的作用。在传代的WNV和SLEV的遗传变化是最小的，但导致增加的相对健身和复制能力的病毒在同源细胞系C6/36蚊子细胞。在异源细胞系DF-1禽类细胞中未测量到类似的增加。这些表型变化与黄病毒中细胞特异性适应的概念一致。(c)2006年爱思唯尔公司All rights reserved.
West Nile Virus (WNV) is a mosquito-borne flavivirus that was introduced into the U.S. in the New York City area in 1999. Despite its successful establishment and rapid spread in a naive environment, WNV has undergone limited evolution since its introduction. This evolutionary stability has been attributed to compromises made to permit alternating cycles of viral replication in vertebrate hosts and arthropod vectors. Outbreaks of a close relative of WNV, St. Louis encephalitis virus (SLEV), occur in the U.S. periodically and are also characterized by limited genetic change overtime. We measured both phenotypic and genotypic changes in WNV and SLEV serially passaged in mosquito cell culture in order to clarify, the role of an individual host cell type in flavivirus adaptation and evolution. Genetic changes in passaged WNV and SLEV were minimal but led to increased relative fitness and replicative ability of the virus in the homologous cell line C6/36 mosquito cells. Similar increases were not measured in the heterologous cell line DF-1 avian cells. These phenotypic changes are consistent with the concept of cell-specific adaptation in flaviviruses. (c) 2006 Elsevier Inc. All rights reserved.