Visualization of fingerprints made easy with dye solution on cellulose membrane
Visualization of fingerprints made easy with dye solution on cellulose membrane
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DOI：
10.1007/s11426-018-9213-x
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发表时间：
2018-02
期刊：
影响因子：
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通讯作者：
J. S. Uppal;Xing-Fang Li;X. Le
中科院分区：
文献类型：
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作者：
J. S. Uppal;Xing-Fang Li;X. Le
指纹识别已经使用了100多年，特别是用于法医调查，个人身份识别和执法[1]。虽然基于试剂的指纹检测自19世纪80年代被引入以来一直在使用，但该技术具有几个缺点，例如使用有害试剂、费力的方案和破坏样品。分析仪器的最新进展使得能够使用复杂的仪器（例如扫描电子显微镜和质谱仪）来检测指纹。然而，这些仪器方法需要训练有素的操作人员，并且成本高昂。在最近的一篇论文中，Wei et al. [2]展示了使用亲水性纤维素膜和染料水溶液的高分辨率和快速检测潜在指纹的方法（图1）。这种方法的基本原理是将染料溶液直接涂在醋酸纤维素膜上的潜在指印的相对亲水的皱纹上。染料水溶液将亲水性纤维素膜染色并掩盖指纹残留物的疏水特性，从而产生负染色的指纹图像。这种膜上指纹的负染色图像允许检测2级细节（如脊分叉和钩）和3级详图（如脊路径偏差和孔隙）通过数据库匹配实现个人身份识别。然后，这些开发的指纹可以直接可视化，并使用相机拍摄的照片进行评估，以识别2级细节，或使用光学显微镜，确定第三级详细信息。此外，这些指纹可以涂上一层金，并使用扫描电子显微镜进行评估，以检测3级细节，包括直径约130 µm的孔。该方法由Wei等人开发。[2]最终允许对潜在指纹的简单和灵敏的检测。这种方法适用于从各种表面提取的指纹，如玻璃，纸箱和陶瓷杯。法医胶带被牢牢地贴在留在感兴趣表面上的潜在指纹上。然后将胶带轻轻剥离并施加到醋酸纤维素膜上，以便将指纹从原始表面转移到醋酸纤维素膜上。然后，采用染料水溶液染色法对指纹进行染色和观察。图像显示了指纹图案的足够细节，无论图像是直接从醋酸纤维素膜获得的还是通过将指纹从玻璃表面转移到醋酸纤维素膜获得的。这种快速的方法需要几秒钟到几分钟的时间来生成负染色的指纹图案，也可以用于检测留在“有问题的表面”上的指纹，例如钞票，人的皮肤和皮革。Wei等人[2]能够改进对从诸如莱亚这样的困难表面上获得的指纹的检测，
Fingerprinting has been used for over 100 years, especially for forensic investigations, personal identification, and law enforcement [1]. Although reagent-based detection of fingerprints has been used since it was introduced in the 1880’s, this technique has several shortcomings, such as the use of harmful reagents, laborious protocols, and the destruction of samples. Recent advances in analytical instrumentation have enabled the detection of fingerprints using sophisticated instruments, such as the scanning electron microscope and mass spectrometer. However, these instrumental methods require trained operators and are costly. In a recent paper, Wei et al.[2] demonstrates a highresolution and rapid detection method for latent fingerprints using a hydrophilic cellulose membrane and dye aqueous solution (Figure 1). The basic principle of this method involves directing the dye solution onto the relatively hydrophilic furrows of a latent finger mark that is placed directly onto a cellulose acetate membrane. The dye aqueous solution stains the hydrophilic cellulose membrane and masks the hydrophobic characteristics of a fingerprint residue, generating a negatively stained image of the fingerprint. This negatively stained image of the fingerprint on the membrane allows for the detection of level 2 details (such as ridge bifurcation and hooks) and level 3 details (such as ridge path deviations and pores) that enable personal identification through database matching.These developed fingermarks can then be directly visualized and evaluated using pictures taken from a camera to identify level 2 details or using an optical microscope to identify the level 3 details. In addition, these fingermarks can be coated with a layer of gold and evaluated using the scanning electron microscope to detect level 3 details, including pores with a diameter of~ 130 µm. This method developed by Wei et al.[2] ultimately allows for simple and sensitive detection of latent fingermarks. This method works well with fingerprints lifted from various surfaces, such as glass, carton, and ceramic cup. A forensic adhesive tape is applied firmly on a latent fingermark that has been left on the surface of interest. The adhesive tape is then gently peeled off and applied onto a cellulose acetate membrane in order to transfer the fingerprint from the original surface to the cellulose acetate membrane. Then, the approach of applying dye aqueous solution is used to stain and view the fingerprint. Images show sufficient details of fingerprint patterns, whether the images are obtained directly from the cellulose acetate membrane or obtained by transferring fingerprints from glass surfaces to the acetate membrane. This quick method, which takes a few seconds to minutes to generate negatively stained patterns of fingerprints, can also be explored for the detection of fingerprints left on “problematic surfaces”, such as bank notes, human skin, and leather. Wei et al.[2] were able to improve on the detection of fingerprints obtained from such difficult surfaces as lea-