Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development
Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development
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DOI：
10.1073/pnas.0805901105
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发表时间：
2008-09-09
影响因子：
11.1
通讯作者：
Zhou, Dao-Xiu
中科院分区：
文献类型：
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作者：
Sun, Qianwen;Zhou, Dao-Xiu
组蛋白赖氨酸甲基化是一种重要的表观遗传修饰，在基因表达中具有激活和抑制作用。含有 Jumonji C (jmjC) 结构域的蛋白质已被证明可以逆转非植物模型系统中的组蛋白甲基化。在这里，我们证明植物 Jumonji C 蛋白与哺乳动物同源物相比既具有保守性又具有特异性。特别是，水稻 JMJD2 家族 jmjC 基因 JMJ706 显示编码富含异染色质的蛋白质。 JMJ706 蛋白在体外特异性逆转组蛋白 H3 (H3K9) 赖氨酸 9 的二甲基化和三甲基化。该基因的功能丧失突变导致 H3K9 的二甲基化和三甲基化增加，并影响小穗发育，包括改变花形态和器官数量。基因表达和组蛋白修饰分析表明 JMJ706 调节花发育调控基因的子集。综上所述，我们的数据表明水稻 JMJ706 编码一种异染色质相关的 H3K9 去甲基酶，参与水稻花发育的调节。
Histone lysine methylation is an important epigenetic modification with both activating and repressive roles in gene expression. Jumonji C (jmjC) domain-containing proteins have been shown to reverse histone methylation in nonplant model systems. Here, we show that plant Jumonji C proteins have both conserved and specific features compared with mammalian homologues. In particular, the rice JMJD2 family jmjC gene JMJ706 is shown to encode a heterochromatin-enriched protein. The JMJ706 protein specifically reverses di- and trimethylations of lysine 9 of histone H3 (H3K9) in vitro. Loss-of-function mutations of the gene lead to increased di- and trimethylations of H3K9 and affect the spikelet development, including altered floral morphology and organ number. Gene expression and histone modification analysis indicates that JMJ706 regulates a subset of flower development regulatory genes. Taken together, our data suggest that rice JMJ706 encodes a heterochromatin-associated H3K9 demethylase involved in the regulation of flower development in rice.