Colorimetric assay for antifungal susceptibility testing of Aspergillus species
Colorimetric assay for antifungal susceptibility testing of Aspergillus species
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DOI：
10.1128/jcm.39.9.3402-3408.2001
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发表时间：
2001-09-01
影响因子：
9.4
通讯作者：
Network, E
中科院分区：
文献类型：
--
作者：
Meletiadis, J;Mouton, JW;Network, E
本文描述了一种基于四氮唑盐2,3-二(2-甲氧基-4-硝基-5-[（亚苯基氨基）羰基]- 2h -四氮唑-氢氧化物（XTT）在甲萘醌作为电子偶联剂存在下还原的抗真菌药敏比色法检测曲霉（烟曲霉、黄曲霉、地曲霉、灰曲霉和霉菌）的方法。200杯XTT/ml与25 muM甲萘醌混合，暴露后2小时内甲酰胺高产，可在孵育24小时后检测到10(2)CFU/ml低接种量形成的菌丝。在此条件下，甲酰胺产量与真菌生物量呈线性相关，两性霉素B和伊曲康唑的浓度效应曲线变化较小。
A colorimetric assay for antifungal susceptibility testing of Aspergillus species (Aspergillus fumigatus, Aspergillus flavus Aspergillus terreis, Aspergillus nidulans, and Aspergillus ustus) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazoliuin-hydroxide (XTT) in the presence of menadione as an electron-coupling agent. The combination of 200 mug of XTT/ml with 25 muM menadione resulted in a high production of formazan within 2 h of exposure, allowing the detection of hyphae formed by low inocula of 10(2) CFU/ml after 24 h of incubation. Under these settings, the formazan production correlated linearly with the fungal biomass and less-variable concentration effect curves for amphotericin B and itraconazole were obtained.