Matrix proteins can generate the higher order architecture of the Golgi apparatus
Matrix proteins can generate the higher order architecture of the Golgi apparatus
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DOI：
10.1038/35039538
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发表时间：
2000-10-26
期刊：
影响因子：
64.8
通讯作者：
Warren, G
中科院分区：
文献类型：
--
作者：
Seemann, J;Jokitalo, E;Warren, G
动物细胞中的高尔基体包含位于细胞中心粒周围且通常位于细胞核旁区域的相互连接的堆叠网(1)。这种细胞器的独特结构被认为取决于细胞骨架 (2) 和细胞质基质蛋白 (3,4)，其中最具特征的是纤维状卷曲螺旋蛋白的高尔金家族和堆叠蛋白的 GRASP 家族 (5-10)。在这里，我们表明这些基质蛋白可以与高尔基体堆栈中的寡糖修饰酶分离，而不影响它们在靠近细胞核的正确位置形成带状网状结构的能力。我们的数据表明，高尔基体是一种结构支架，可以独立于改变转运货物的含酶膜而存在，但通常由其填充。高尔基体的这一新概念进一步表明，高尔基体可能是一种自主细胞器，而不是与内质网处于简单动态平衡的细胞器。
The Golgi apparatus in animal cells comprises a reticulum of linked stacks in the pericentriolar and often in the juxtanuclear regions of the cell(1). The unique architecture of this organelle is thought to depend on the cytoskeleton(2) and cytoplasmic matrix proteins(3,4)-the best characterized being the golgin family of fibrous, coiled-coil proteins and the GRASP family of stacking proteins(5-10). Here we show that these matrix proteins can be separated from oligosaccharide-modifying enzymes in the Golgi stack without affecting their ability to form a ribbon-like reticulum in the correct location near to the nucleus. Our data suggest that the Golgi is a structural scaffold that can exist independently of, but is normally populated by, the enzyme-containing membranes that modify transiting cargo. This new concept of the Golgi further indicates that the Golgi may be an autonomous organelle rather than one that is in simple dynamic equilibrium with the endoplasmic reticulum.