EFFECTS OF BETA-LACTAM ANTIBIOTICS ON PEPTIDOGLYCAN SYNTHESIS IN GROWING NEISSERIA-GONORRHOEAE, INCLUDING CHANGES IN THE DEGREE OF O-ACETYLATION
EFFECTS OF BETA-LACTAM ANTIBIOTICS ON PEPTIDOGLYCAN SYNTHESIS IN GROWING NEISSERIA-GONORRHOEAE, INCLUDING CHANGES IN THE DEGREE OF O-ACETYLATION
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DOI：
10.1128/jb.147.2.633-641.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
PERKINS, HR
中科院分区：
文献类型：
--
作者：
BLUNDELL, JK;PERKINS, HR
低浓度的β-内酰胺类抗生素引起生长中的N.淋病培养物中存在的SDS可溶性聚合物的（少量）量没有明显变化。对照细胞中SDS不溶性产物仅部分被卵白色溶菌酶溶解（约40%），但可全部被Chalaropsis B胞壁酶释放。在暴露于β-对溶菌酶敏感的标记肽聚糖的比例增加到60%。通过TLC检查Chalaropsis B菌株表明，它们含有具有和不具有O-乙酰化的二糖-肽单体和具有1或2个O-乙酰基或不具有O-乙酰基的双二糖-肽二聚体。β-内酰胺类抗生素引起O-乙酰化程度的降低，但对肽聚糖交联的量没有很大影响。它们还具有扩大细菌以及保存和增厚隔膜的作用，这些隔膜可以在EM下的薄切片中观察到。这些结果与β-γ的影响之间的关系内酰胺对醚化N.淋病是讨论。
Low concentrations of .beta.-lactam antibiotics caused an increased uptake of radioactive glucosamine into the sodium dodecyl sulfate[SDS]-insoluble peptidoglycan of growing N. gonorrhoeae. There was no appreciable change in the (small) amount of SDS-soluble polymer present in the cultures. The SDS-insoluble product in control cells was only partially dissolved by egg white lysozyme (about 40%) but could all be released by the Chalaropsis B muramidase. In cells exposed to .beta.-lactams the proportion of labeled peptidoglycan susceptible to lysozyme increased to 60%. Examination of the Chalaropsis B digests by TLC showed that they contained disaccharide-peptide monomers with and without O-acetylation and bis-disaccharide-peptide dimers with 1 or 2 O-acetyl groups or with none. .beta.-Lactam antibiotics caused a decrease in the degree of O-acetylation but did not greatly affect the amount of peptidoglycan cross-linking. They also had the effect of enlarging the bacteria and conserving and thickening the septa that could be observed in thin sections under the EM. The relationship between these results and the effects of .beta.-lactams on in vitro synthesis of peptidoglycan by ether-treated N. gonorrhoeae is discussed.