Imaging of lipid species by MALDI mass spectrometry
Imaging of lipid species by MALDI mass spectrometry
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DOI：
10.1194/jlr.r800051-jlr200
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发表时间：
2009-04-01
影响因子：
6.5
通讯作者：
Barkley, Robert M.
中科院分区：
文献类型：
--
作者：
Murphy, Robert C.;Hankin, Joseph A.;Barkley, Robert M.
MALDI的最新进展使直接检测存在于细胞膜中的完整分子物种脂质成为可能。当在涂有MALDI基质的组织表面获得连续光谱时，直接分析薄组织切片会产生丰富的与脂质相关的离子。由于脂类等疏水分子中存在大量共价结合的氢原子，这些离子存在着显著的质量缺陷，因此脂类衍生的离子通常可以与其他生物分子区分开来。分子离子的碰撞活化可以用来确定脂质家族，通常从结构上定义分子物种。给出了检测磷脂、鞘脂和甘油脂的具体例子，以及小鼠脑和肾组织切片的图像。许多不同的脂质分子种类和Na+和K+附着离子的区域分布通常定义了组织内的解剖区域。用MALDI质谱仪对脂类进行成像。J.Lipid Res.2009年。S317-S322。
Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the direct analysis of thin tissue slices when sequential spectra are acquired across a tissue surface that has been coated with a MALDI matrix. The lipid-derived ions can often be distinguished from other biomolecules because of the significant mass defect that these ions present due to the large number of covalently bound hydrogen atoms in hydrophobic molecules such as lipids. Collisional activation of the molecular ions can be used to determine the lipid family and often structurally define the molecular species. Specific examples in the detection of phospholipids, sphingolipids, and glycerolipids are presented with images of mouse brain and kidney tissue slices. Regional distribution of many different lipid molecular species and Na+ and K+ attachment ions often define anatomical regions within the tissues.-Murphy, R. C., J. A. Hankin, and R. M. Barkley. Imaging of lipid species by MALDI mass spectrometry. J. Lipid Res. 2009. S317-S322.