Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves
Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves
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DOI：
10.1016/j.vetmic.2008.11.012
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发表时间：
2009-05-12
影响因子：
3.3
通讯作者：
Saegerman, C.
中科院分区：
文献类型：
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作者：
Dal Pozzo, F.;De Clercq, K.;Saegerman, C.
牛通常在自然或实验感染蓝舌病病毒（BTV）后发生亚临床感染。BTV血清型8（BTV-8）在欧洲的引入以感染牛的临床症状表现为特征。为了研究BTV-8在该宿主中的发病机制，需要能够再现该疾病的临床表现的动物模型。在这项工作中，两只小牛皮下和静脉注射低传代细胞适应株BTV-8。两只小牛均显示典型的蓝舌病临床体征，包括发热、眼分泌物、结膜炎、口腔粘膜充血、唇和舌溃疡和坏死性病变、下颌下水肿、冠状部和部水肿。根据病变的严重程度进行评分，每天和实验结束时计算每只动物的临床总评分。这两个小牛成为病毒血症感染后24小时和血清转化发生在7和11天P.I.在这项研究中，我们提出了一个方案的发展，能够重现与BTV-8在现场条件下观察到的病变的严重程度小牛感染。(C)2008 Elsevier B. V.保留所有权利。
Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the manifestation of clinical signs in infected cattle. In order to study the pathogenesis of BTV-8 in this host, an animal model able to reproduce the clinical manifestations of the disease is required. In this work, two calves were subcutaneously and intravenously injected with a low passage cell-adapted strain of BTV-8. Both calves showed typical bluetongue clinical signs, including pyrexia, ocular discharge, conjunctivitis, oral mucosal congestion, development of ulcers and necrotic lesions on the lips and tongue, submandibular oedema, coronitis and oedema of the coronet and pastern region. A score was assigned depending on the severity of the lesions and a total clinical score was calculated for each animal daily and at the end of the experiment. Both calves became viraemic 24 h post-infection and seroconversion occurred between 7 and 11 days P.I.In this study we present the development of a protocol of infection in calves able to reproduce the severity of the lesions observed with BTV-8 in field conditions. (C) 2008 Elsevier B.V. All rights reserved.