The impact of hibernation and arousal on energy metabolism and antioxidant defenses in leech (Whitmania pigra)
The impact of hibernation and arousal on energy metabolism and antioxidant defenses in leech (Whitmania pigra)
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冬眠和觉醒对水蛭能量代谢和抗氧化防御的影响（Whitmania Pigra）
DOI：
10.1111/are.13447
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发表时间：
2018
影响因子：
2
通讯作者：
Manjun Wu
中科院分区：
文献类型：
--
作者：
Jia wang;Hongzhuan Shi;Qiaosheng Guo;Fei Liu;Shimeng Yan;Daoxin Dai;Manjun Wu
水母是一种重要的药用资源，在亚洲水产养殖业中广泛养殖，长期冬眠期间体重下降严重影响水产养殖系统的生产效率。我们在冬眠-唤醒的循环中检测了冬眠者(W.npgra)肠道的能量代谢和抗氧化物酶。研究结果表明，越冬的特点是在深度冬眠期间能量代谢显著下降，并被重新升温的觉醒所中断。在复温觉醒过程中，能量代谢显著增加。对能量代谢的调节抑制可能有助于能量节约。氧化应激在深度冬眠期间随着氧化代谢的降低而减少，但在进入冬眠和从冬眠中唤醒时增加。这种在唤醒过程中抗氧化防御(AD)的上调被解释为保护肠道免受氧化损伤，伴随着从冬眠中唤醒的代谢活动的极大增加，以及进入冬眠期间AD的上调被解释为水蚤暴露在显著的应激(冷驯化)下，必须适当地处理这些压力，以避免不可逆转的组织损伤。可以得出这样的结论：白纹伊蚊具有强大的AD系统，在进入冬眠和觉醒期间都能保护它免受自由基的伤害。这些结果表明，可以通过中断或缩短冬眠来提高水蚤的生产效率，这是一种冬眠适应机制。
Whitmania pigra is an important medicinal resource that is widely farmed in aquaculture in Asia, and a decrease in body weight occurring during hibernation and hibernation lasting long time has serious impacts on production efficiency in aquaculture system. We examined energy metabolic and antioxidant enzymes of intestine from a hibernator (W. pigra) over cycles of hibernation-arousal. Results of the study demonstrated that hibernation in W. pigra was characterized by a profound decrease in energy metabolic during deep hibernation that was interrupted by rewarming arousal. And energy metabolic increased significantly during the rewarming arousal. Regulated suppression of energy metabolism probably contributes to energy savings. Oxidative stress decreased during deep hibernation along with a reduction in oxidative metabolism, but increased during entrance into hibernation and arousal from hibernation. This up-regulation of antioxidant defense (AD) during arousal was interpreted as protection of the intestine against oxidative damage to come with the enormous increase in metabolic activity during arousal from hibernation, and the up-regulation of AD during entrance into hibernation was interpreted that leech are exposed to significant stresses (cold acclimation) that must be dealt with appropriately to avoid irreversible tissue damage. It can be concluded that W. pigra has a strong AD system that protects it from the injurious effects of free radicals either during periods of entrance into hibernation and arousal. These results indicate the adaptive mechanism of hibernation that may be applied to increase production efficiency of leech by interrupting or shortening hibernation.