Regulation of intracellular trafficking of human CD1d by association with MHC class II molecules
Regulation of intracellular trafficking of human CD1d by association with MHC class II molecules
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DOI：
10.1093/emboj/21.7.1650
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发表时间：
2002-04-02
期刊：
影响因子：
11.4
通讯作者：
Cresswell, P
中科院分区：
文献类型：
--
作者：
Kang, SJ;Cresswell, P
CD 1家族成员是能够将细菌或合成糖脂呈递给T细胞的抗原呈递分子。在这里，我们表明，一个子集的人CD 1d分子与主要组织相容性复合体（MHC）II类分子，无论是在细胞表面上，并在后期的内体/溶酶体车厢II类分子在运输过程中瞬时积累。的相互作用是在内质网与II类不变链复合物，并似乎保持整个II类贩运途径。缺乏其细胞质YXXZ内化基序的截短形式的CD 1d在II类分子存在下被转运至晚期内体/溶酶体隔室。此外，相同的CD 1d缺失突变体通过转染靶向表达II类分子和不变链的HeLa细胞中的溶酶体隔室。在仅表达p33形式的不变链的HeLa细胞中的溶酶体隔室中也发现了缺失突变体。这些数据表明，CD 1d的细胞内运输途径可能会被II类分子和不变链在炎症过程中诱导改变。
CD1 family members are antigen-presenting molecules capable of presenting bacterial or synthetic glycolipids to T cells. Here we show that a subset of human CD1d molecules are associated with major histocompatibility complex (MHC) class II molecules, both on the cell surface and in the late endosomal/lysosomal compartments where class II molecules transiently accumulate during transport. The interaction is initiated in the endoplasmic reticulum with class II-invariant chain complexes and appears to be maintained throughout the class II trafficking pathway. A truncated form of CD1d which lacks its cytoplasmic YXXZ internalization motif is transported to late endosomal/lysosomal compartments in the presence of class II molecules. Furthermore, the same CD1d deletion mutant is targeted to lysosomal compartments in HeLa cells expressing class II molecules and invariant chain by transfection. The deletion mutant was also found in lysosomal compartments in HeLa cells expressing only the p33 form of the invariant chain. These data suggest that the intracellular trafficking pathway of CD1d may be altered by class II molecules and invariant chain induced during inflammation.