Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage φPVL carrying Panton-Valentine leukocidin genes
Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage φPVL carrying Panton-Valentine leukocidin genes
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DOI：
10.1016/s0378-1119(98)00278-9
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发表时间：
1998-07-17
期刊：
影响因子：
3.5
通讯作者：
Kamio, Y
中科院分区：
文献类型：
--
作者：
Kaneko, J;Kimura, T;Kamio, Y
从丝裂霉素诱导的金黄色葡萄球菌V8 （ATCC 49775）中分离出的葡萄球菌Panton-Valentine leukocidin （PVL）基因[lukS-PV-lukF-PV]存在于噬菌体phi PVL的基因组中（Kaneko， J., Kimura， T., Kawakami， Y., Tomita， T., Kamio， Y., 1997）。从丝裂霉素c处理的金黄色葡萄球菌V8 （ATCC 49775）分离的噬菌体样颗粒中的潘通-瓦伦丁嗜白蛋白基因。Biosci。Biotechnol。生物化学。61,1960-1962)。在这项研究中，我们分析了phi PVL基因组的完整核苷酸序列，并确定了phi PVL特异位点整合到宿主染色体所需的att位点（attL、attR和attB）。该基因组全长41 401 bp，具有9个碱基的3'错开内聚端（cos），包含63个orf，通过氨基酸序列的比较和从phi PVL颗粒中分离的蛋白质分析，初步鉴定出参与DNA复制的调节蛋白、结构蛋白、holin、lysin、整合酶和dUTPase。phi PVL的[lukS-PV-lukF-PV]、attP和int（整合酶基因）按顺序位于基因组上4.0 kb片段内，位置非常接近，该片段位于左cos位点和右cos位点的中间位置。此外，attP区含有5个直接重复序列，与其他一些金黄色葡萄球菌噬菌体的重组酶结合位点高度同源。phi PVL基因组被发现整合到一个ORF中，该ORF编码包含725个氨基酸残基的未知蛋白质，具有两个亮氨酸拉链状蛾。(C) 1998 Elsevier Science B.V.版权所有
The staphylococcal Panton-Valentine leukocidin (PVL) genes, [lukS-PV-lukF-PV], exist on the genome of a temperate bacteriophage phi PVL isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775) (Kaneko, J., Kimura, T., Kawakami, Y., Tomita, T., Kamio, Y., 1997b. Panton-Valentine leukocidin genes in phage-like particle isolated from mitomycin C-treated Staphylococcus aureus V8 (ATCC 49775). Biosci. Biotechnol. Biochem. 61, 1960-1962). In this study, the complete nucleotide sequence of the phi PVL genome was analyzed, and the att sites (attL, attR, and attB) required for site-specific integration of phi PVL into the host chromosome were also determined. The linear double-stranded phi PVL genome comprised 41 401 bp with 3' staggered cohesive ends (cos) of nine bases and contained 63 ORFs, among which the regulatory proteins involved in DNA replication, structural proteins, a holin, a lysin, an integrase, and dUTPase, were tentatively identified by the comparison of the deduced amino acid sequences and by the analysis of the proteins isolated from phi PVL particles. The [lukS-PV-lukF-PV], attP, and int (integrase gene) of phi PVL were all located very close to one another within a 4.0-kb segment on the genome in the order given, and this segment was located at the center from the left and the right cos sites. In addition, the attP region contained five direct repeat sequences that showed a high degree of homology with the recombinase-binding sites of some other S. aureus bacteriophages. The phi PVL genome was found to integrate into an ORF encoding an unknown protein comprising 725 amino acid residues with two leucine zipper-like moths. (C) 1998 Elsevier Science B.V. All rights reserved.