Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes
Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes
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DOI：
10.1007/s11262-008-0293-2
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发表时间：
2009-02-01
期刊：
影响因子：
1.6
通讯作者：
Bishop, Richard P.
中科院分区：
文献类型：
--
作者：
Gallardo, Carmina;Mwaengo, Dufton M.;Bishop, Richard P.
对67株欧洲、美洲、西非和东非猪瘟病毒（ASFV）分离株的p54基因进行全序列测序，结果显示，根据p72部分序列，西非和欧洲猪瘟病毒分离株属于优势基因型I，根据p54序列可分为4个主要亚型。这突出了p54基因测序作为ASFV病毒分型的一种额外的、中等分辨率的分子流行病学工具的价值。我们进一步评估了基于p54的基因分型，结合其他两个基因的部分序列，以确定导致肯尼亚疾病暴发的病毒的遗传关系和起源。在2006年和2007年在家猪中暴发严重出血性疾病之后，使用基于p72基因的PCR检测证实来自肯尼亚西部和中部的动物感染了非洲猪瘟病毒。从巨噬细胞培养中分离出11株吸血病毒，采用p54基因全长测序、p72基因部分测序和B602L基因可变区四聚体氨基酸重复区分析进行基因分型。数据显示，这些分离株的p72和p54序列与导致2003年乌干达非洲猪瘟暴发的病毒相同。引起2007年肯尼亚第二次暴发的肯尼亚分离株B602L序列中四聚体重复序列的数量略有不同。肯尼亚和乌干达病毒分离株遗传相似性的一个实际含义是，非洲猪瘟控制需要一种区域方法。
Complete sequencing of p54-gene from 67 European, American, and West and East African Swine Fever virus (ASFV) isolates revealed that West African and European ASFV isolates classified within the predominant Genotype I according to partial sequencing of p72 were discriminated into four major sub-types on the basis of their p54 sequences. This highlighted the value of p54 gene sequencing as an additional, intermediate-resolution, molecular epidemiological tool for typing of ASFV viruses. We further evaluated p54-based genotyping, in combination with partial sequences of two other genes, for determining the genetic relationships and origin of viruses responsible for disease outbreaks in Kenya. Animals from Western and central Kenya were confirmed as being infected with ASFV using a p72 gene-based PCR assay, following outbreaks of severe hemorrhagic disease in domestic pigs in 2006 and 2007. Eleven hemadsorbing viruses were isolated in macrophage culture and genotyped using a combination of full-length p54-gene sequencing, partial p72-gene sequencing, and analysis of tetrameric amino acid repeat regions within the variable region of the B602L gene (CVR). The data revealed that these isolates were identical in their p72 and p54 sequence to viruses responsible for ASF outbreaks in Uganda in 2003. There was a minor difference in the number of tetrameric repeats within the B602L sequence of the Kenyan isolates that caused the second Kenyan outbreak in 2007. A practical implication of the genetic similarity of the Kenyan and Ugandan viral isolates is that ASF control requires a regional approach.