Use of nitrocellulose membranes as a scaffold in cell culture
Use of nitrocellulose membranes as a scaffold in cell culture
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DOI：
10.1007/s10616-012-9458-x
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发表时间：
2013-01-01
期刊：
影响因子：
2.2
通讯作者：
Zhang, Yali
中科院分区：
文献类型：
--
作者：
Li, Aimin;Wang, Yadong;Zhang, Yali
硝化纤维素膜是最重要和最古老的纤维素衍生物之一，通常用于研究和诊断应用中的核酸和蛋白质检测。然而，有限数量的研究探索了它们是否可以作为细胞生长的支架。在这项研究中，我们研究了这种聚合物材料支持人类细胞生长的能力。八个已建立的细胞系进行了检查，粘附，生长，扩散，并通过光学显微镜后，苏木精和伊红和/或免疫细胞化学染色和扫描电子显微镜的硝酸纤维素膜上的生存。细胞凋亡和乳酸脱氢酶（LDH）的泄漏也进行了评估。所有的细胞都很容易粘附和扩散在硝酸纤维素膜以及盖玻片的表面上，并且细胞保持消化系统特异性基因的表达。在硝酸纤维素膜上生长的细胞的凋亡或LDH的泄漏没有检测到显着的变化。这些结果表明，硝酸纤维素膜对人体细胞具有良好的细胞相容性，可用于组织工程支架。此外，我们证明了与显微镜成像特定相关的硝化纤维素的额外和未充分利用的性质，因为它可以呈现几乎透明的，因此在染色后可以在光学显微镜下直接观察生长在这种膜上的细胞。
Nitrocellulose membranes, one of the most important and oldest cellulose derivatives, are commonly used for nucleic acid and protein detection in research and diagnostic applications. However, a limited number of studies have explored whether they can act as scaffolds for cell growth. In this study, we investigated this polymeric material for its ability to support the growth of human cells. Eight established cell lines were examined for adherence, growth, spread, and survival on nitrocellulose membranes by optical microscopy after hematoxylin and eosin and/or immunocytochemical staining and by scanning electron microscopy. Apoptosis and leakage of lactate dehydrogenase (LDH) were also assessed. All cells readily adhered to and spread on the surface of nitrocellulose membranes as well as coverslips, and the cells maintained the expression of digestive system-specific genes. No significant change was detected in apoptosis or leakage of LDH from cells grown on nitrocellulose membranes. These results suggested that nitrocellulose membranes have a suitable cytocompatibility towards human cells and that they might be used for tissue-engineering scaffolds. Moreover, we demonstrate an additional and underused property of nitrocellulose of specific relevance to microscopic imaging, as it can be rendered virtually transparent, thus the cells growing on such membranes can be observed directly under an optical microscope after staining.