Immunogenic glycoproteins of laboratory and vaccine strains of Varicella-Zoster virus.
Immunogenic glycoproteins of laboratory and vaccine strains of Varicella-Zoster virus.
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水痘带状疱疹病毒实验室和疫苗株的免疫原性糖蛋白。
DOI：
10.1128/iai.31.3.1044-1053.1981
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发表时间：
1981
影响因子：
3.1
通讯作者：
Friedrichs,WE
中科院分区：
文献类型：
--
作者：
Grose,C;Edmond,BJ;Friedrichs,WE
用豚鼠和家兔制备了抗两株水痘-带状疱疹病毒（VZV）的高滴度抗血清：VZV-32（低传代实验室株）和VZV-Oka（经人和豚鼠胚胎细胞传代减毒的疫苗株）。当用动物VZV免疫血清以及人带状疱疹血清从VZV感染的细胞中沉淀放射性标记的糖蛋白，并通过聚丙烯酰胺凝胶电泳和荧光照相术分析免疫沉淀物时，观察到用任一菌株感染的细胞培养物具有相似的电泳图谱，其含有分子量约为62，000、98，000和118的主要糖蛋白，000.一个突出的高分子量（约150，000）nonglycosylated多肽在两种菌株中也被确定。这些决定因素都证明了间接（葡萄球菌蛋白A抗体吸附剂）和直接免疫沉淀，只要VZV免疫血清抗体效价大于或等于1：128使用。进一步分析单个豚鼠VZV抗血清显示出一定的异质性，这似乎与免疫方法有关，而不是病毒特异性抗体的水平。用完全弗氏佐剂乳化的VZV提取物引起对所有主要免疫原性病毒糖蛋白的抗体应答，而在初次免疫期间单独接种病毒的豚鼠最初产生VZV抗体，该抗体未能沉淀最高分子量的糖蛋白（gp 118）。因此，弗氏型佐剂促进VZV免疫后，在远交豚鼠的体液免疫应答的成熟。
High-titered antisera were prepared in guinea pigs and rabbits against two strains of varicella-zoster virus (VZV): VZV-32, a low-passage laboratory strain, and VZV-Oka, a vaccine strain attenuated by passage in both human and guinea pig embryo cells. When the animal VZV-immune sera, as well as a human zoster serum, were used to precipitate radiolabeled glycoproteins from VZV-infected cells and the immune precipitates were analyzed by polyacrylamide gel electrophoresis and fluorography, it was observed that cell cultures infected with either strain had similar electrophoretic profiles containing major glycoproteins of approximate molecular weights 62,000, 98,000, and 118,000. A prominent high-molecular-weight (approximately 150,000) nonglycosylated polypeptide was identified in both strains also. These determinants were demonstrable by both indirect (staphylococcal protein A-antibody adsorbent) and direct immunoprecipitation, as long as VZV-immune sera with an antibody titer greater than or equal to 1:128 were used. Further analysis of individual caviid VZV antisera demonstrated some heterogeneity which appeared to be related to the method of immunization rather than the level of virus-specific antibody. VZV extracts emulsified with complete Freund adjuvant elicited an antibody response to all major immunogenic viral glycoproteins, whereas guinea pigs inoculated with virus alone during the primary immunization initially produced VZV antibody which failed to precipitate the highest-molecular-weight glycoprotein (gp118). Thus, Freund-type adjuvants promoted the maturation of the humoral immune response after VZV immunization in outbred guinea pigs.