Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.
Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.
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DOI：
10.1016/j.devcel.2011.05.008
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发表时间：
2011-06-14
影响因子：
11.8
通讯作者：
Millar, Jonathan B. A.
中科院分区：
文献类型：
--
作者：
Meadows, John C.;Shepperd, Lindsey A.;Vanoosthuyse, Vincent;Lancaster, Theresa C.;Sochaj, Alicja M.;Buttrick, Graham J.;Hardwick, Kevin G.;Millar, Jonathan B. A.
纺锤体检查点是主要的细胞周期控制机制，确保姐妹染色单体在后期发生之前是双向的。极光B激酶是染色体乘客复合物的催化亚基，它既能使不产生张力的动粒附着不稳定，又能同时维持纺锤体检查点信号。然而，目前还不清楚检查点是如何在染色体双向定位后沉默的。我们证明了1型磷酸酶（PP 1Dis 2）与Spc 7的N-末端和Klp 5-Klp 6（驱动蛋白-8）复合物的非马达结构域两者的关联对于抵消极光B激酶以有效地沉默纺锤体检查点是必要的。Klp 5和Klp 6在检查点沉默中的作用对这类驱动蛋白是特异性的，并且独立于它们的运动活性。这些数据表明，至少需要两个不同的PP 1池，一个动粒相关，另一个电机相关，沉默纺锤体检查点。
The spindle checkpoint is the prime cell cycle control mechanism that ensures sister chromatids are bi-oriented before anaphase takes place. Aurora B kinase, the catalytic subunit of the chromosome passenger complex, both destabilises kinetochore attachments that do not generate tension and simultaneously maintains the spindle checkpoint signal. However, it is unclear how the checkpoint is silenced following chromosome bi-orientation. We demonstrate that association of type 1 phosphatase (PP1Dis2) to both the N-terminus of Spc7 and the non-motor domains of the Klp5-Klp6 (Kinesin-8) complex are necessary to counteract Aurora B kinase to efficiently silence the spindle checkpoint. The role of Klp5 and Klp6 in checkpoint silencing is specific to this class of kinesin and independent of their motor activities. These data demonstrate that at least two distinct pools of PP1, one kinetochore associated and the other motor associated, are needed to silence the spindle checkpoint.
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DOI：
10.1016/j.cub.2009.06.043
发表时间：
2009-07-28
期刊：
Current biology : CB
影响因子：
--
作者：
Pinsky BA;Nelson CR;Biggins S
通讯作者：
Biggins S
影响因子：
21.3
作者：
Gupta, Mohan L., Jr.;Carvalho, Pedro;Pellman, David
通讯作者：
Pellman, David
影响因子：
10.5
作者：
Gassmann, Reto;Holland, Andrew J.;Desai, Arshad
通讯作者：
Desai, Arshad
影响因子：
9.2
作者：
Mayr, Monika I.;Huemmer, Stefan;Mayer, Thomas U.
通讯作者：
Mayer, Thomas U.
DOI：
10.1083/jcb.201001006
发表时间：
2010-03-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Liu D;Vleugel M;Backer CB;Hori T;Fukagawa T;Cheeseman IM;Lampson MA
通讯作者：
Lampson MA