Evaluation of gravimetric techniques to estimate the microvascular filtration coefficient.
Evaluation of gravimetric techniques to estimate the microvascular filtration coefficient.
复制标题
评估用于估计微血管过滤系数的重量分析技术。
DOI：
10.1152/ajpregu.00342.2010
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Quick,CM
中科院分区：
文献类型：
--
作者：
Dongaonkar,RM;Laine,GA;Stewart,RH;Quick,CM
Microvascular permeability to water is characterized by the microvascular filtration coefficient (Kf). Conventional gravimetric techniques to estimateKfrely on data obtained from either transient or steady-state increases in organ weight in response to increases in microvascular pressure. Both techniques result in considerably different estimates and neither account for interstitial fluid storage and lymphatic return. We therefore developed a theoretical framework to evaluateKfestimation techniques by1) comparing conventional techniques to a novel technique that includes effects of interstitial fluid storage and lymphatic return,2) evaluating the ability of conventional techniques to reproduceKffrom simulated gravimetric data generated by a realistic interstitial fluid balance model,3) analyzing new data collected from rat intestine, and4) analyzing previously reported data. These approaches revealed that the steady-state gravimetric technique yields estimates that are not directly related toKfand are in some cases directly proportional to interstitial compliance. However, the transient gravimetric technique yields accurate estimates in some organs, because the typical experimental duration minimizes the effects of interstitial fluid storage and lymphatic return. Furthermore, our analytical framework reveals that the supposed requirement of tying off all draining lymphatic vessels for the transient technique is unnecessary. Finally, our numerical simulations indicate that our comprehensive technique accurately reproduces the value ofKfin all organs, is not confounded by interstitial storage and lymphatic return, and provides corroboration of the estimate from the transient technique.
