Spatial patterning of cholinergic amacrine cells in the mouse retina.
Spatial patterning of cholinergic amacrine cells in the mouse retina.
复制标题
小鼠视网膜中胆碱能无长突细胞的空间模式。
DOI：
10.1002/cne.21630
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Reese,BenjaminE
中科院分区：
文献类型：
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作者：
Whitney,IreneE;Keeley,PatrickW;Raven,MaryA;Reese,BenjaminE
小鼠视网膜内核层（INL）和神经节细胞层（GCL）的两类胆碱能无分泌细胞在空间组织上存在差异，但这种差异的基础尚不清楚。本研究在六种不同胆碱能细胞数量的小鼠中研究了这一问题，解决了这些细胞的规律性、包装和间距如何随着菌株、层数和密度的变化而变化。GCL细胞的胆碱能细胞数量均低于INL细胞。GCL的最近邻和Voronoi域正则性指数以及填充因子均持续降低。虽然这些规则性指标和填充系数在密度变化中基本稳定，但对于GCL和INL来说，有效半径与密度呈负相关，在GCL中更小，变化更大。因此，尽管GCL的密度较低，但相邻的细胞比高密度的INL更有可能彼此靠近，从而降低了规律性和堆积性。胆碱能细胞在空间组织上的这种差异可能是由于在发育过程中，GCL中的细胞被视轴突束和扩张的视网膜血管被动地移位。为了支持这一解释，我们展示了胆碱能体相对于树突茎的位移，以及出生后发育过程中包装效率和规律性的下降，这对GCL来说更为严重。中国生物医学工程学报，2008,31(2):444 - 444。©2008 Wiley‐Liss, Inc。
The two populations of cholinergic amacrine cells in the inner nuclear layer (INL) and the ganglion cell layer (GCL) differ in their spatial organization in the mouse retina, but the basis for this difference is not understood. The present investigation examined this issue in six strains of mice that differ in their number of cholinergic cells, addressing how the regularity, packing, and spacing of these cells varies as a function of strain, layer, and density. The number of cholinergic cells was lower in the GCL than in the INL in all six strains. The nearest neighbor and Voronoi domain regularity indexes as well as the packing factor were each consistently lower for the GCL. While these regularity indexes and the packing factor were largely stable across variation in density, the effective radius was inversely related to density for both the GCL and INL, being smaller and more variable in the GCL. Consequently, despite the lower densities in the GCL, neighboring cells were more likely to be positioned closer to one another than in the higher‐density INL, thereby reducing regularity and packing. This difference in the spatial organization of cholinergic cells may be due to the cells in the GCL having been passively displaced by fascicles of optic axons and an expanding retinal vasculature during development. In support of this interpretation, we show such displacement of cholinergic somata relative to their dendritic stalks and a decline in packing efficiency and regularity during postnatal development that is more severe for the GCL. J. Comp. Neurol. 508:1–12, 2008. © 2008 Wiley‐Liss, Inc.