Comparative analyses of microbial structures and gene copy numbers in the anaerobic digestion of various types of sewage sludge.
Comparative analyses of microbial structures and gene copy numbers in the anaerobic digestion of various types of sewage sludge.
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DOI：
10.1016/j.biortech.2017.12.097
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发表时间：
2018-01
影响因子：
11.4
通讯作者：
T. Hidaka;I. Tsushima;J. Tsumori
中科院分区：
文献类型：
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作者：
T. Hidaka;I. Tsushima;J. Tsumori
多种污泥的厌氧共消化是一种很有前途的能量回收方法，但该过程比单一消化污泥更复杂。微生物结构分析和基因定量的适用性，以了解微生物的条件进行了评价。结果表明，基因分析的信息是有用的，除了常规参数，如总固体，pH值和沼气生产管理厌氧共消化和受损的微生物。总细菌16S rRNA基因拷贝数是评估污水污泥不稳定厌氧消化的最有用的工具，而不是mcrA和总古细菌16S rRNA基因拷贝数，以及高通量测序。在pH故障期间基因拷贝数的一级衰减速率高于稳定操作中微生物的典型衰减速率。测序分析，包括多维标度，显示了非常不同的微生物结构变化，但结果并不一致。
Anaerobic co-digestion of various sewage sludges is a promising approach for greater recovery of energy, but the process is more complicated than mono-digestion of sewage sludge. The applicability of microbial structure analyses and gene quantification to understand microbial conditions was evaluated. The results show that information from gene analyses is useful in managing anaerobic co-digestion and damaged microbes in addition to conventional parameters like total solids, pH and biogas production. Total bacterial 16S rRNA gene copy numbers are the most useful tools for evaluating unstable anaerobic digestion of sewage sludge, rather than mcrA and total archaeal 16S rRNA gene copy numbers, and high-throughput sequencing. First order decay rates of gene copy numbers during pH failure were higher than typical decay rates of microbes in stable operation. The sequencing analyses, including multidimensional scaling, showed very different microbial structure shifts, but the results were not consistent.