A transient RNA interference assay system using Arabidopsis protoplasts
A transient RNA interference assay system using Arabidopsis protoplasts
复制标题
DOI：
10.1271/bbb.67.2674
复制
发表时间：
2003-12-01
影响因子：
1.6
通讯作者：
Kobayashi, A
中科院分区：
文献类型：
--
作者：
An, CI;Sawada, A;Kobayashi, A
双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在真核生物中诱导序列特异性基因沉默。RNAi现在被用作许多真核生物（包括植物）功能基因组学的有力工具。本文报道了一种利用拟南芥叶肉细胞和悬浮培养细胞（细胞系T87）的原生质体构建的dsRNA介导的瞬时RNAi检测系统。将dsRNA引入原生质体导致靶转基因的显著沉默。本实验为模式植物拟南芥原生质体中RNAi的诱导提供了一种简便、有效的方法。
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.