Antifungal effect of gamma irradiation and sodium dichloroisocyanurate against Penicillium expansum on pears
Antifungal effect of gamma irradiation and sodium dichloroisocyanurate against Penicillium expansum on pears
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DOI：
10.1111/lam.12466
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发表时间：
2015-11
影响因子：
2.4
通讯作者：
R. Jeong;eun-Hee CHu;Eun-Jung Shin;E. Lee;Youn-Sig Kwak;H.-J. Park
中科院分区：
文献类型：
--
作者：
R. Jeong;eun-Hee CHu;Eun-Jung Shin;E. Lee;Youn-Sig Kwak;H.-J. Park
研究了γ射线照射（GI）对梨果实扩展青霉菌的体外和体内抗真菌活性。GI对扩展青霉孢子萌发、芽管伸长和菌丝生长均有抑制作用，其中以1·8 kGy的抑制作用最强。GI以剂量依赖性方式影响分生孢子的膜完整性和细胞渗漏。菌丝体蛋白质和糖的渗漏量随剂量的增加而沿着增加。在较低剂量下与氯供体二氯-s-三嗪三酮钠（NaDCC）组合评价GI，以检查对扩展疟原虫的抑制。有趣的是，只有0.2 kGy的GI和70 ppm的NaDCC的组合处理表现出显着的协同抗真菌活性。复合处理降低梨果实蓝霉病的机制可能与破坏病原菌细胞膜，导致菌丝细胞质物质损失有关。
Gamma irradiation (GI) was evaluated for its in vitro and in vivo antifungal activity against Penicillium expansum on pear fruits. GI showed a complete inhibition of spore germination, germ tube elongation and mycelial of P. expansum, especially 1·8 kGy. GI affected the membrane integrity and cellular leakage of conidia in a dose‐dependent manner. Furthermore, the leakage of protein and sugar from mycelia increased along with the dose. GI was evaluated at lower doses in combination with a chlorine donor, sodium dichloro‐s‐triazinetrione (NaDCC), to examine the inhibition of P. expansum. Interestingly, only a combined treatment with 0·2 kGy of GI and 70 ppm of NaDCC exhibited significant synergistic antifungal activity. The mechanisms by which the combined treatment decreased the blue mould decay of pear fruits could directly associated with the disruption of the cell membrane of the fungal pathogen, resulting in a loss of cytoplasmic materials from the hyphae.