Purification of vanadium binding substance from the blood cells of the tunicate, Ascidia sydneiensis samea.

Purification of vanadium binding substance from the blood cells of the tunicate, Ascidia sydneiensis samea.
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从被囊动物海鞘 (Ascidia sydneiensis Samea) 的血细胞中纯化钒结合物质。

DOI:
10.1016/s0006-291x(86)80361-8
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发表时间:
1986
影响因子:
3.1
通讯作者:
H. Sakurai
H. Sakurai
中科院分区:
生物学4区
文献类型:
--
作者:
H. Michibata;T. Miyamoto;H. Sakurai

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在pH2.3条件下,通过SephadexG-25和SE-纤维素柱层析,对钒结合物进行了部分纯化。结合物无色,相对稳定,保持钒离子。该物质中的钒离子以氧钒形式(VO(IV))存在。此外,该物质对外源性钒离子(V)具有明显的亲和力,并含有还原糖。
Vanadium binding substance has been partially purified through chromatographies on Sephadex G-25 and SE-Cellulose at pH 2.3. The binding substance was colorless, relatively stable and maintained vanadium ion. The vanadium ion in the substance existed in vanadyl form (VO(IV)). Furthermore, the substance had an apparent affinity for exogenous vanadium ion(V) and contained a reducing sugar.