Syntheses and copper(II)-dependent DNA photocleavage by acridine and anthracene 1,10-phenanthroline conjugate systems.
Syntheses and copper(II)-dependent DNA photocleavage by acridine and anthracene 1,10-phenanthroline conjugate systems.
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DOI：
10.1039/b502485d
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发表时间：
2005-05
影响因子：
3.2
通讯作者：
Lourdes Gude;María-José Fernández;K. Grant;A. Lorente
中科院分区：
文献类型：
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作者：
Lourdes Gude;María-José Fernández;K. Grant;A. Lorente
我们报道了一系列基于1,10-菲罗啉在2位和9位共价连接到两个苯、萘、吖啶或蒽的发色团的化合物的合成和表征。在紫外线（pH = 7.0, 22℃，350 nm）照射下，吖啶和蒽衍生物可以有效地切割pUC19质粒DNA。此外，在DNA光解反应中加入Cu2+可以显著提高光裂解水平。有趣的是，当蒽类化合物的浓度从35微米降低到0.25微米时，观察到相反的趋势。在铜（II）的存在下，DNA的光分裂明显减少。
We report the syntheses and characterization of a series of compounds based on 1,10-phenanthroline covalently tethered, at the 2 and 9 positions, to either two benzene, naphthalene, acridine or anthracene chromophores. The acridine and anthracene derivatives are shown to efficiently cleave pUC19 plasmid DNA upon irradiation with ultraviolet light (pH = 7.0, 22 degrees C, 350 nm). Furthermore, photocleavage levels are markedly increased by the addition of Cu2+ to the DNA photolysis reactions. Interestingly, when the concentrations of the anthracene compounds are lowered from 35 microM to 0.25 microM, the reverse trend is observed. DNA photocleavage is markedly reduced in the presence of copper(II).