Essential role for puma in development of postembryonic neural crest-derived cell lineages in zebrafish
Essential role for puma in development of postembryonic neural crest-derived cell lineages in zebrafish
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DOI：
10.1016/s0012-1606(03)00016-2
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发表时间：
2003-04-15
影响因子：
2.7
通讯作者：
Parker, NB
中科院分区：
文献类型：
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作者：
Parichy, DM;Turner, JM;Parker, NB
多能神经嵴干细胞已在妊娠晚期的胚胎中被鉴定。然而，关于这些细胞在胚后发育过程中产生、维持和招募的机制仍然存在重大问题。斑马鱼，Danio rerio，提供了一个机会，以确定这些过程中必不可少的基因，通过筛选突变体的特征可能依赖于这些细胞在变态和成年生活。其中一个特征是由神经嵴衍生的色素细胞或色素细胞形成的色素模式，包括黑色黑色素细胞、黄色黄色素细胞和虹彩虹膜细胞。以往的分析表明，成年斑马鱼的色素模式依赖于从头分化的潜伏前体细胞在早期和晚期阶段的色素模式变态。为了更好地了解这些细胞的发育，在这项研究中，我们分析了斑马鱼美洲狮突变体，该突变体消除了大部分在变态过程中分化的成年黑色素细胞，但保留了在胚胎发生过程中分化的完整的早期幼虫黑色素细胞。我们使用上位性分析表明，美洲狮促进发展的早期出现的变质黑色素细胞依赖于kit受体酪氨酸激酶，以及后期出现的变质黑色素细胞依赖于G蛋白偶联内皮素受体b1（ednrb1）和kit相关的fms受体酪氨酸激酶。我们进一步证明，在色素模式变态，美洲狮突变体有缺陷的细胞表达转录试剂盒，ednrb1和fms，以及HMG结构域转录因子sox10的数量。由于美洲狮突变表型是温度敏感的，我们使用温度变化实验，以确定一个关键时期的美洲狮活动在色素图案变态。最后，我们用细胞移植来证明美洲狮的行为细胞自主地促进扩张的色素细胞谱系在变态。这些结果表明，斑马胚胎后发育过程中的神经嵴干细胞的谱系多样化的模型。(C)2003 Elsevier Science（美国）。All rights reserved.
Multipotent neural crest stem cells have been identified in late gestation amniote embryos. Yet, significant questions remain about the mechanisms by which these cells are generated, maintained, and recruited during postembryonic development. The zebrafish, Danio rerio, offers an opportunity to identify genes essential for these processes, by screening for mutants with defects in traits likely to depend on these cells during metamorphosis and adult life. One such trait is the pigment pattern formed by neural crest-derived pigment cells, or chromatophores, which include black melanophores, yellow xanthophores, and iridescent iridophores. Previous analyses have demonstrated that the adult zebrafish pigment pattern depends on the de novo differentiation of latent precursor cells during both early and late phases of pigment pattern metamorphosis. To better understand the development of these cells, in this study, we analyze the zebrafish puma mutant, which ablates most of the adult melanophores that differentiate during metamorphosis, but leaves intact early larval melanophores that differentiate during embryogenesis. We use epistasis analyses to show that puma promotes the development of both early-appearing metamorphic melanophores that depend on the kit receptor tyrosine kinase, as well as late-appearing metamorphic melanophores that depend on both the G-protein-coupled endothelin receptor b1 (ednrb1) and the kit-related fms receptor tyrosine kinase. We further demonstrate that, during pigment pattern metamorphosis, puma mutants have deficiencies in the numbers of cells expressing transcripts for kit, ednrb1, and fms, as well as the HMG domain transcription factor sox10. Because the puma mutant phenotype is temperature-sensitive, we use temperature-shift experiments to identify a critical period for puma activity during pigment pattern metamorphosis. Finally, we use cell transplantations to show that puma acts cell-autonomously to promote the expansion of pigment cell lineages during metamorphosis. These results suggest a model for the lineage diversification of neural crest stem cells during zebratish postembryonic development. (C) 2003 Elsevier Science (USA). All rights reserved.