ANTIVIRAL ACTIVITY OF PROSTAGLANDIN-A ON ENCEPHALOMYOCARDITIS VIRUS-INFECTED CELLS - A UNIQUE EFFECT UNRELATED TO INTERFERON
ANTIVIRAL ACTIVITY OF PROSTAGLANDIN-A ON ENCEPHALOMYOCARDITIS VIRUS-INFECTED CELLS - A UNIQUE EFFECT UNRELATED TO INTERFERON
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DOI：
10.1099/0022-1317-66-11-2355
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
JACOBSEN, H
中科院分区：
文献类型：
--
作者：
ANKEL, H;MITTNACHT, S;JACOBSEN, H
A系列前列腺素（PGAs）对仙台病毒、牛痘病毒和水疱性口炎病毒的抗病毒作用已有报道，并提示PGAs的抗病毒作用与干扰素之间的关系。我们研究了PGAs对脑心肌炎（EMC）病毒的抗病毒活性。通过对病毒复制的单周期试验，我们的结果表明，PGAs仅在细胞感染后存在于培养基中时才具有抑制作用，并且在感染后3至5小时的潜伏期内最有效。此外，在PGA处理的感染细胞中，病毒RNA合成被阻断，因此，感染后5小时细胞细胞质中的病毒抗原大大减少。由于PGAs的抗病毒作用不受放线菌素D的干扰，当细胞RNA合成大大减少时，诱导新的细胞蛋白似乎不太可能是PGAs具有抗病毒活性的原因。在单独的实验中，我们无法直接证明干扰素或两种dsrna依赖性酶（2',5' -寡腺苷酸合成酶和蛋白激酶）的诱导作用，这两种酶在干扰素细胞中大大增加。因此，我们得出结论，PGAs的抗病毒活性与干扰素的抗病毒作用无关，并且涉及一种独立于细胞蛋白合成的独特机制。
Antiviral effects of prostaglandins of the A series (PGAs) on Sendai, vaccinia and vesicular stomatitis viruses have previously been reported and a relationship between the antiviral actions of PGAs and interferons has been suggested. We have investigated the antiviral activity of PGAs on encephalomyocarditis (EMC) virus. Using single-cycle assays of virus replication our results indicate that PGAs only inhibit when present in the culture medium after the cells are infected, and that they are most effective during incubation periods including from 3 to 5 h post-infection. Furthermore, viral RNA synthesis is blocked in infected cells treated with PGA and, as a result, viral antigens are greatly reduced in the cytoplasm of the cells 5 h post-infection. Since the antiviral effect of PGAs is unperturbed by actinomycin D, when cellular RNA synthesis is greatly reduced, it appears unlikely that induction of new cellular proteins is the reason for the antiviral activity of PGAs. In separate experiments we were unable to demonstrate directly the induction of interferon, or of the two dsRNA-dependent enzymes, 2'',5''-oligoadenylate synthetase and protein kinase, which are greatly increased in interferon-cells. Thus, we conclude that the antiviral activity of PGAs is unrelated to the antiviral action of interferons and involves a unique mechanism independent of cellular protein synthesis.