Molecular imaging of oncolytic viral therapy.
Molecular imaging of oncolytic viral therapy.
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DOI：
10.1038/mto.2014.7
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Fong Y
中科院分区：
文献类型：
--
作者：
Haddad D;Fong Y
溶瘤病毒作为一种潜在的治疗选择已经在癌症世界中留下了印记，由于更高的肿瘤选择性，其可能的优点是减少副作用和加强治疗效果。结果是如此有希望，溶瘤病毒治疗现在已经在几个国家被批准进行临床试验。然而，临床研究可能受益于通过分子成像非侵入性和连续识别病毒靶向位点的能力，以提供安全性、有效性和毒性信息。此外，溶瘤病毒治疗的分子成像可以提供更敏感和特异性的诊断技术来检测肿瘤起源，更重要的是，转移的存在。已经研究了几种用于病毒复制的分子成像的策略，这些策略大致分为光学和深层组织成像，利用编码荧光蛋白、条件酶和膜蛋白和转运蛋白的几种报告基因。各种成像方法有助于分子成像，包括计算机断层扫描、磁共振成像、正电子发射断层扫描、单光子发射CT、γ闪烁扫描和光声成像。此外，几种分子探针用于医学成像，其充当靶向部分或信号传导剂。本文将探讨复制型溶瘤病毒治疗的体内分子成像的临床前和临床应用。
Oncolytic viruses have made their mark on the cancer world as a potential therapeutic option, with the possible advantages of reduced side effects and strengthened treatment efficacy due to higher tumor selectivity. Results have been so promising, that oncolytic viral treatments have now been approved for clinical trials in several countries. However, clinical studies may benefit from the ability to noninvasively and serially identify sites of viral targeting via molecular imaging in order to provide safety, efficacy, and toxicity information. Furthermore, molecular imaging of oncolytic viral therapy may provide a more sensitive and specific diagnostic technique to detect tumor origin and, more importantly, presence of metastases. Several strategies have been investigated for molecular imaging of viral replication broadly categorized into optical and deep tissue imaging, utilizing several reporter genes encoding for fluorescence proteins, conditional enzymes, and membrane protein and transporters. Various imaging methods facilitate molecular imaging, including computer tomography, magnetic resonance imaging, positron emission tomography, single photon emission CT, gamma-scintigraphy, and photoacoustic imaging. In addition, several molecular probes are used for medical imaging, which act as targeting moieties or signaling agents. This review will explore the preclinical and clinical use of in vivo molecular imaging of replication-competent oncolytic viral therapy.