A nutrient-permeable channel on the intraerythrocytic malaria parasite.
A nutrient-permeable channel on the intraerythrocytic malaria parasite.
复制标题
红细胞内疟原虫上的营养可渗透通道。
DOI：
10.1002/9780470515730.ch7
复制
发表时间：
1999
影响因子：
--
通讯作者：
S. Desai
中科院分区：
文献类型：
--
作者：
S. Desai
红细胞内疟疾寄生虫恶性疟原虫从血清中获取营养物质时至少面临三个膜屏障：人红细胞膜、寄生液泡膜（PVM）和寄生虫质膜。 PVM 是一种特殊的寄生虫膜，可将寄生虫与红细胞胞质分离。我使用膜片钳方法来识别和表征 PVM 上的主要传输路径。在成熟的游离滋养体上形成的千兆欧姆密封揭示了 PVM 上的阳离子和阴离子可渗透的 140 pS 通道。该通道以高密度存在，在 PVM 静息电位下 95% 以上的时间处于开放状态，并且能够跨 PVM 运输氨基酸和单糖。然后，这种营养物质可渗透的通道被重构为人工脂质双层，它表现出相似的斜率电导、门控、电压依赖性和对可溶性营养物质的渗透性。在双层中，该通道被发现具有非饱和动力学和 23 A 的有效孔径。这些实验与膜片钳的发现一起表明，高容量分子筛可以使寄生虫从红细胞胞浆中获取可溶性营养物质。
The intraerythrocytic malaria parasite Plasmodium falciparum faces at least three membranous barriers to acquisition of nutrients from serum: the human erythrocyte membrane, the parasitophorous vacuole membrane (PVM) and the parasite plasma membrane. The PVM is a specialized parasite-derived membrane that separates the parasite from erythrocyte cytosol. I used the patch clamp method to identify and characterize the main transport pathway on the PVM. Gigaohm seals, formed on mature freed trophozoites, revealed a 140 pS channel permeable to both cations and anions on the PVM. This channel is present at high density, is open more than 95% of the time at the PVM resting potential, and is capable of transporting amino acids and monosaccharides across the PVM. This nutrient-permeable channel was then reconstituted into artificial lipid bilayers, where it exhibited similar slope conductances, gating, voltage dependence and permeability to soluble nutrients. In bilayers, the channel was found to have non-saturating kinetics and an effective pore diameter of 23 A. These experiments, together with the patch clamp findings, suggest a high capacity molecular sieve that allows the parasite to acquire soluble nutrients from erythrocyte cytosol.
DOI：
10.1073/pnas.91.2.509
发表时间：
1994-01-18
影响因子：
11.1
作者：
SCHWAB, JC;BECKERS, CJM;JOINER, KA
通讯作者：
JOINER, KA
无红细胞疟疾寄生虫的体外分泌测定。
影响因子：
--
作者：
Haldar,K;Elmendorf,HG;Das,A;Li,WL;Ferguson,DJ;Elford,BC
通讯作者：
Elford,BC