Chitin oligosaccharides as candidate patterning agents in zebrafish embryogenesis.
Chitin oligosaccharides as candidate patterning agents in zebrafish embryogenesis.
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DOI：
10.1387/ijdb.10794076
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发表时间：
2000-02
期刊：
影响因子：
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通讯作者：
C. Semino;M. Allende
中科院分区：
文献类型：
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作者：
C. Semino;M. Allende
在这项工作中，我们研究了在斑马鱼（Danio rerio）发育过程中产生的n -乙酰壳寡糖（naco）的可能功能。首先，我们发现naco是在斑马鱼的早期胚胎发育过程中在体内合成的。其次，我们证明了向单细胞期胚胎注射纯细菌几丁质酶会引起发育缺陷，严重影响发育胚胎的后躯干和尾部。此外，一种内源性几丁质酶活性在细胞内和细胞外都被检测到，表明细胞可能将其分泌到细胞外空间。此外，这种区隔化似乎在功能上与细胞外抑制有关，而不是细胞内抑制，内源性几丁质酶活性导致与注射几丁质酶63的胚胎相似的形态缺陷。最后，对斑马鱼ZDG42基因的表达进行了分析，该基因被认为参与了naco的合成。转录本在囊胚晚期、原肠胚形成期间被检测到，并在体细胞发生过程中以前后波的形式在近中胚层中表达。
In this work we investigate the possible function of N-acetyl-chitooligosaccharides (NACOs) produced during zebrafish (Danio rerio) development. First, we show that NACOs are synthesized in vivo during early embryogenesis in the zebrafish. Second, we demonstrate that injection of a pure bacterial chitinase into one-cell stage embryos elicits developmental defects in which the posterior trunk and tail of developing embryo are severely affected. In addition, an endogenous chitinase activity detected both intra- and extracellularly is described, suggesting that cells may secrete it into the extracellular space. Moreover, this compartmentalization appears to be functionally relevant as inhibition of the extracellular, but not the intracellular, endogenous chitinase activity causes morphological defects similar to those seen in embryos injected with chitinase 63. Finally, analysis of the expression of the zebrafish ZDG42 gene, which has been suggested to be involved in synthesis of NACOs, is described. Transcripts are detected from late blastula stage, during gastrulation, and move as an anterior-posterior wave of expression in adaxial mesoderm during somitogenesis.