Coexistence of Wolbachia with Buchnera aphidicola and a secondary symbiont in the aphid Cinara cedri
Coexistence of Wolbachia with Buchnera aphidicola and a secondary symbiont in the aphid Cinara cedri
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DOI：
10.1128/jb.186.19.6626-6633.2004
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发表时间：
2004-10-01
影响因子：
3.2
通讯作者：
Latorre, A
中科院分区：
文献类型：
--
作者：
Gómez-Valero, L;Soriano-Navarro, M;Latorre, A
细胞内共生现象在昆虫界非常普遍。对于蚜虫Cinara雪松，我们已经确定了通过电子显微镜三个共生细菌，其特征在于它们不同的大小，形态和电密度。16 S rDNA基因的PCR扩增和测序结果表明，除了蚜虫Buchnera aphidicola外，蚜虫的主要内共生体C.雪松含有一种次级共生体（S共生体），该共生体先前被发现与蚜虫（PASS或R型）和一种属于沃尔巴克氏体属的α-变形杆菌有关。使用特定的细菌探针设计的共生体16 S rDNA序列的原位杂交，我们已经表明，沃尔巴克氏体是由周围的S共生体只有几分钟的细菌。此外，观察到的B.蚜虫和S共生体具有相似的大小，并且被安置在单独的特定细菌细胞（细菌细胞）中。有趣的是，与迄今为止研究的所有蚜虫的情况相反，S共生体显示出占据与B相似大小甚至更大的细菌细胞空间。蚜虫。这些发现，沿着的事实，C。雪松港的B。最小的细菌基因组和S共生菌感染所有Cinara属。分析到目前为止，建议在这些物种中的细菌替代的可能性。
Intracellular symbiosis is very common in the insect world. For the aphid Cinara cedri, we have identified by electron microscopy three symbiotic bacteria that can be characterized by their different sizes, morphologies, and electrodensities. PCR amplification and sequencing of the 16S ribosomal DNA (rDNA) genes showed that, in addition to harboring Buchnera aphidicola, the primary endosymbiont of aphids, C. cedri harbors a secondary symbiont (S symbiont) that was previously found to be associated with aphids (PASS, or R type) and an alpha-proteobacterium that belongs to the Wolbachia genus. Using in situ hybridization with specific bacterial probes designed for symbiont 16S rDNA sequences, we have shown that Wolbachia was represented by only a few minute bacteria surrounding the S symbionts. Moreover, the observed B. aphidicola and the S symbionts had similar sizes and were housed in separate specific bacterial cells, the bacteriocytes. Interestingly, in contrast to the case for all aphids examined thus far, the S symbionts were shown to occupy a similarly sized or even larger bacteriocyte space than B. aphidicola. These findings, along with the facts that C. cedri harbors the B. aphidicola strain with the smallest bacterial genome and that the S symbionts infect all Cinara spp. analyzed so far, suggest the possibility of bacterial replacement in these species.