Investigation of the antimicrobial properties of modified multilayer diamond-like carbon coatings on 316 stainless steel
Investigation of the antimicrobial properties of modified multilayer diamond-like carbon coatings on 316 stainless steel
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DOI：
10.1016/j.surfcoat.2016.11.035
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发表时间：
2017-03
影响因子：
5.4
通讯作者：
Shaun Robertson;D. Gibson;W. Mackay;S. Reid;Craig Williams;R. Birney
中科院分区：
文献类型：
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作者：
Shaun Robertson;D. Gibson;W. Mackay;S. Reid;Craig Williams;R. Birney
采用脉冲(直流)空心阴极等离子体增强化学气相沉积(HC-PECVD)技术在直径25 mm的316不锈钢盘片上沉积了改性类金刚石(DLC)涂层。沉积了总厚度为1-2μm的多层膜，包括掺锗和不掺锗。通过扫描电子显微镜/能谱分析、表面能/接触角分析和可能的生物膜抑制性能评估，对涂层进行了表征。改性类金刚石和掺锗类金刚石(Ge-DLC)涂层均显示出显著的抗生物污损作用。铜绿假单胞菌是一种革兰氏阴性杆菌。降低了90%的INP。与对照相比，DLC和Ge-DLC都观察到了铜绿假单体量，然而这种影响不能单独归因于Ge的掺入。修饰后的DLC和GE-DLC对NS均无明显抑制作用。金黄色葡萄球菌是一种革兰氏阳性细菌。扫描电子显微镜OFP。在Ge-DLC涂层的316不锈钢上的铜绿假单胞膜清楚地显示了细胞壁的破坏和细胞成分的泄漏；这种影响在改性DLC涂层中没有观察到。这表明，掺Ge的DLC涂层可能表现出一种与P相反的杀伤性作用模式。铜绿假单胞菌。
Modified diamond-like carbon (DLC) coatings were deposited onto 25 mm diameter 316 stainless steel discs by pulsed (direct current) hollow cathode plasma enhanced chemical vapour deposition (HC-PECVD). Multilayer films of total thickness 1–2 μm were deposited, both with and without germanium dopant. Characterisation of the coatings was performed by SEM/EDX, surface energy/contact angle analysis, and assessment of possible biofilm-inhibiting properties. Both modified DLC and germanium-doped DLC (Ge-DLC) coatings showed a significant anti-biofouling effect onP. aeruginosa, a Gram-negative bacterium. A 90% reduction inP. aeruginosabiomass was observed compared to control for both DLC and Ge-DLC, however this effect could not be attributed to germanium incorporation alone. Neither modified DLC nor Ge-DLC showed a significant inhibitory effect onS. aureus, a Gram-positive bacterium. Scanning electron microscopy ofP. aeruginosabiofilms on Ge-DLC coated 316 stainless steel clearly displayed disruption of the cellular wall, as well as leakage of cellular components; this effect was not observed with modified DLC coating. This suggests that germanium-doped DLC coatings may potentially exhibit a cidal mode of action versusP. aeruginosabiofilms.