The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)
The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)
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DOI：
10.1073/pnas.1110681108
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发表时间：
2011-10-11
影响因子：
11.1
通讯作者：
Yang, Changhuei
中科院分区：
文献类型：
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作者：
Zheng, Guoan;Lee, Seung Ah;Yang, Changhuei
我们报告了一种芯片级无透镜宽视场显微成像技术，亚像素透视扫描显微镜，它可以自动呈现生长或汇合细胞培养物的显微图像。我们证明，该技术可用于构建智能培养皿平台，称为ePetri，用于细胞培养实验。这种技术利用了最近广泛和廉价的高性能图像传感器芯片的可用性，以提供低成本和自动化的显微镜解决方案。与两种主要的无透镜显微镜方法，光流体显微镜和数字同轴全息显微镜不同，这种新方法完全能够与细胞培养物或细胞可能连续连接的任何样品一起工作。通过我们的原型，我们展示了在660 nm分辨率下对面积为6 mm x 4 mm的样品进行成像的能力。作为进一步的证明，我们表明，该方法可以应用于图像彩色染色的细胞培养样品和图像和跟踪细胞培养生长直接在培养箱内。最后，我们证明了这种方法可以在整个传感器表面上跟踪胚胎干细胞分化。基于该技术的智能培养皿可以通过减少人力和污染风险来显着简化和改进细胞培养实验。
We report a chip-scale lensless wide-field-of-view microscopy imaging technique, subpixel perspective sweeping microscopy, which can render microscopy images of growing or confluent cell cultures autonomously. We demonstrate that this technology can be used to build smart Petri dish platforms, termed ePetri, for cell culture experiments. This technique leverages the recent broad and cheap availability of high performance image sensor chips to provide a low-cost and automated microscopy solution. Unlike the two major classes of lensless microscopy methods, optofluidic microscopy and digital in-line holography microscopy, this new approach is fully capable of working with cell cultures or any samples in which cells may be contiguously connected. With our prototype, we demonstrate the ability to image samples of area 6 mm x 4 mm at 660-nm resolution. As a further demonstration, we showed that the method can be applied to image color stained cell culture sample and to image and track cell culture growth directly within an incubator. Finally, we showed that this method can track embryonic stem cell differentiations over the entire sensor surface. Smart Petri dish based on this technology can significantly streamline and improve cell culture experiments by cutting down on human labor and contamination risks.