Cosuppression of RBCS3B in Arabidopsis leads to severe photoinhibition caused by ROS accumulation
Cosuppression of RBCS3B in Arabidopsis leads to severe photoinhibition caused by ROS accumulation
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DOI：
10.1007/s00299-014-1597-4
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发表时间：
2014-03
影响因子：
6.2
通讯作者：
Gaomiao Zhan;Rong-Jun Li;Zhi-yong Hu;Jing Liu;Linbin Deng;Shi-you Lu;Wei Hua
中科院分区：
文献类型：
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作者：
Gaomiao Zhan;Rong-Jun Li;Zhi-yong Hu;Jing Liu;Linbin Deng;Shi-you Lu;Wei Hua
Rubisco小亚基基因RBCS 3的共抑制导致了线粒体的白化病或苍白绿色表型，这些表型是由ROS积累引起的。尽管如此，它的功能仍然没有被彻底理解。本论文通过构建一个驱动表达Rubisco小亚基基因（senseRBCS 3B）的转基因载体，获得了4个具有白化病和淡绿色绿色表型的转基因株系（RBCS 3B-7、18、33和35）。在这些转基因株系中产生的表型被发现是由共抑制引起的。在这些品系中，RBCS 3B-7在正常光照下生长时表现出最严重的表型，包括高度降低、发育停滞和开花前植株死亡。在整个发育过程中，RBCS 3B-7的叶肉细胞中叶绿体数量减少，叶绿体中堆积的类囊体逐渐被分解。此外，RBCS 3B-7细胞系具有光敏感性，PSII活性测定表明，即使在正常光照下，RBCS 3B-7细胞也受到严重的光抑制。结果表明，RBCS 3B-7的光抑制是由于ROS的积累，加速了PSII的光损伤，抑制了PSII的修复。
Key messageCosuppression of anArabidopsisRubisco small subunit geneRBCS3BatArabidopsisresulted in albino or pale green phenotypes which were caused by ROS accumulationAbstractAs the most abundant protein on Earth, Rubisco has received much attention in the past decades. Even so, its function is still not understood thoroughly. In this paper, fourArabidopsistransgenic lines (RBCS3B-7,18,33, and 35) with albino or pale green phenotypes were obtained by transformation with a construct driving expression of senseRBCS3B, a Rubisco small subunit gene. The phenotypes produced in these transgenic lines were found to be caused by cosuppression. Among these lines,RBCS3B-7displayed the most severe phenotypes including reduced height, developmental arrest and plant mortality before flowering when grown under normal light on soil. Chloroplast numbers in mesophyll cells were decreased compared to WT, and stacked thylakoids of chloroplasts were broken down gradually inRBCS3B-7throughout development. In addition, theRBCS3B-7line was light sensitive, and PSII activity measurement revealed thatRBCS3B-7suffered severe photoinhibition, even under normal light. We found that photoinhibition was due to accumulation of ROS, which accelerated photodamage of PSII and inhibited the repair of PSII inRBCS3B-7.