Genomic regulation of Krüppel-like-factor family members by corticosteroid receptors in the rat brain.
Genomic regulation of Krüppel-like-factor family members by corticosteroid receptors in the rat brain.
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DOI：
10.1016/j.ynstr.2023.100532
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发表时间：
2023-03
影响因子：
5
通讯作者：
Reul, Johannes M. H. M.
中科院分区：
文献类型：
--
作者：
Kennedy, Clare L. M.;Price, Emily M.;Mifsud, Karen R.;Salatino, Silvia;Sharma, Eshita;Engledow, Simon;Broxholme, John;Goss, Hannah M.;Reul, Johannes M. H. M.
海马矿物皮质激素受体（MRs）和糖皮质激素受体（GRs）在海马中介导糖皮质激素（GC）的作用。这些受体与靶基因中的糖皮质激素反应元件（GREs）结合，在应激和昼夜变化下引发转录效应。直到最近，人们对生理条件下海马MRs和gr的全基因组靶点知之甚少。根据我们对大鼠海马的全基因组MR和GR ChIP-seq和Ribo-Zero RNA-seq研究，我们研究了kr<s:1> ppel样因子（KLFs）在昼夜节律变化和急性应激后作为整个大脑MR和GR的靶点。特别是，已知Klf2， Klf9和Klf15对应激和/或GC有反应，并在包括突触可塑性和神经元分化在内的神经生物学过程中发挥作用。我们发现，急性应激后，海马、杏仁核、前额叶皮层和新皮层的Klf2、Klf9和Klf15中MR和GR与GREs的结合增加，导致昼夜变化，并伴有相应的hnRNA和mRNA水平上调。肾上腺切除术消除了特定Klf基因的转录上调。这些结果表明，MRs和GRs在暴露于急性应激或响应昼夜节律变化后，可能与其他转录因子一起调节整个大脑的Klf基因表达。
Hippocampal mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs) mediate glucocorticoid hormone (GC) action in the hippocampus. These receptors bind to glucocorticoid responsive elements (GREs) within target genes, eliciting transcriptional effects in response to stress and circadian variation. Until recently, little was known about the genome-wide targets of hippocampal MRs and GRs under physiological conditions. Following on from our genome-wide MR and GR ChIP-seq and Ribo-Zero RNA-seq studies on rat hippocampus, we investigated the Krüppel-like factors (KLFs) as targets of MRs and GRs throughout the brain under circadian variation and after acute stress. In particular, Klf2, Klf9 and Klf15 are known to be stress and/or GC responsive and play a role in neurobiological processes including synaptic plasticity and neuronal differentiation. We found increased binding of MR and GR to GREs within Klf2, Klf9 and Klf15 in the hippocampus, amygdala, prefrontal cortex, and neocortex after acute stress and resulting from circadian variation, which was accompanied by upregulation of corresponding hnRNA and mRNA levels. Adrenalectomy abolished transcriptional upregulation of specific Klf genes. These results show that MRs and GRs regulate Klf gene expression throughout the brain following exposure to acute stress or in response to circadian variation, likely alongside other transcription factors.
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影响因子：
4.7
作者：
Chandramohan, Yalini;Droste, Susanne K.;Reul, Johannes M. H. M.
通讯作者：
Reul, Johannes M. H. M.
影响因子：
4.8
作者：
Denver, RJ;Ouellet, L;Puymirat, J
通讯作者：
Puymirat, J
影响因子：
5.3
作者：
Mizoguchi, K;Ishige, A;Tabira, T
通讯作者：
Tabira, T
影响因子：
4.8
作者：
Bagamasbad, Pia;Ziera, Tim;Denver, Robert J.
通讯作者：
Denver, Robert J.
DOI：
10.1073/pnas.0803216105
发表时间：
2008-08-19
影响因子：
11.1
作者：
Kolber, Benedict J.;Roberts, Marie S.;Muglia, Louis J.
通讯作者：
Muglia, Louis J.