Comprehensive on-line LC/LC/MS of proteins
Comprehensive on-line LC/LC/MS of proteins
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DOI：
10.1021/ac961155l
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发表时间：
1997-04-15
影响因子：
7.4
通讯作者：
Anderegg, RJ
中科院分区：
文献类型：
--
作者：
Opiteck, GJ;Lewis, KC;Anderegg, RJ
这是一个全面的二维液相色谱（LC）系统的描述，用于分离蛋白质混合物。该系统采用阳离子交换色谱法和反相色谱法（RPLC）。两个LC系统由一个配备两个存储回路的八端口阀耦合，并在计算机控制下。通过紫外检测器和电喷雾质谱仪对RPLC出水进行采样。通过这种方式，大分子的复杂混合物可以在不到2小时内快速分离、脱盐和分析分子量。该系统的实用性通过标准物和大肠杆菌细胞裂解液的混合物得到了证明。
This is a description of a comprehensive two-dimensional liquid chromatography (LC) system for the separation of protein mixtures. This system uses cation-exchange chromatography followed by reversed-phase chromatography(RPLC). The two LC systems are coupled by an eight-port valve equipped with two storage loops and under computer control. The RPLC effluent is sampled by both a UV detector and an electrospray mass spectrometer. In this way, complex mixtures of large biomolecules can be rapidly separated, desalted, and analyzed for molecular weight in less than 2 h. The system's utility is demonstrated with a mixture of standards and an Escherichia coli cell lysate.