Activation of Nervous System Development Genes in Bone Marrow Derived Mesenchymal Stem Cells Following Spaceflight Exposure
Activation of Nervous System Development Genes in Bone Marrow Derived Mesenchymal Stem Cells Following Spaceflight Exposure
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DOI：
10.1002/jcb.22765
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发表时间：
2010-10-01
影响因子：
4
通讯作者：
Cancedda, Ranieri
中科院分区：
文献类型：
--
作者：
Monticone, Massimiliano;Liu, Yi;Cancedda, Ranieri
前体骨细胞的细胞分裂停滞和成骨细胞功能降低被认为是太空飞行期间观察到的微重力诱导的骨丢失的原因。然而，潜在的分子机制仍然没有解开。在克服了太空使命中飞行细胞相关的技术困难后，我们首次报道了2006年3月30日至4月8日在空间使命ISS 12 S（联盟TMA-8+增量13）上的KUBIK内飞行的潜在成骨小鼠骨髓基质细胞（BMSC）在3D培养系统中的行为（实验“基质-2”）。Flight 1g对照培养物在位于有效载荷内的离心机中进行。地面控制在地球上的另一个KUBIK有效载荷和培养皿中维持。一半的培养物用骨诱导培养基刺激。总RNA提取的差异表明，细胞增殖抑制飞行样品。Affyestival技术显示，1，599个基因在太空飞行暴露后改变了表达。细胞周期基因的表达减少证实了空间中细胞增殖的抑制。出乎意料的是，大多数被调节的表达被发现在与神经发育、神经元形态发生、神经冲动传递和突触的各种过程相关的基因中，这提出了BMSC中谱系限制的问题。J.细胞。111：442-452，2010. (C)2010 Wiley-Liss，Inc.
Stalled cell division in precursor bone cells and reduced osteoblast function are considered responsible for the microgravity-induced bone loss observed during spaceflight. However, underlying molecular mechanisms remain unraveled. Having overcome technological difficulties associated with flying cells in a space mission, we present the first report on the behavior of the potentially osteogenic murine bone marrow stromal cells (BMSC) in a 3D culture system, flown inside the KUBIK aboard space mission ISS 12S (Soyuz TMA-8+Increment 13) from March 30 to April 8, 2006 (experiment "Stroma-2''). Flight 1g control cultures were performed in a centrifuge located within the payload. Ground controls were maintained on Earth in another KUBIK payload and in Petri dishes. Half of the cultures were stimulated with osteo-inductive medium. Differences in total RNA extracted suggested that cell proliferation was inhibited in flight samples. Affymetrix technology revealed that 1,599 genes changed expression after spaceflight exposure. A decreased expression of cell-cycle genes confirmed the inhibition of cell proliferation in space. Unexpectedly, most of the modulated expression was found in genes related to various processes of neural development, neuron morphogenesis, transmission of nerve impulse and synapse, raising the question on the lineage restriction in BMSC. J. Cell. Biochem. 111: 442-452, 2010. (C) 2010 Wiley-Liss, Inc.