ZmERF21 directly regulates hormone signaling and stress‐responsive gene expression to influence drought tolerance in maize seedlings
ZmERF21 directly regulates hormone signaling and stress‐responsive gene expression to influence drought tolerance in maize seedlings
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ZmERF21 直接调节激素信号传导和胁迫响应基因表达，从而影响玉米幼苗的耐旱性
DOI：
10.1111/pce.14243
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Lixia Ku
中科院分区：
文献类型：
--
作者：
Zhiyong Wang;Xiang Zhao;Zhenzhen Ren;Salah Fatouh Abou‐Elwafa;Xiaoyu Pu;Yingfang Zhu;D;an Dou;Huihui Su;Haiyang Cheng;Zhixue Liu;Yanhui Chen;Ertao Wang;Ruixin Shao;Lixia Ku
干旱胁迫对作物发育和产量产生不利影响。玉米在其生命周期中经常遭受干旱胁迫。提高玉米的耐旱性是玉米育种的重点。本研究发现了一个与玉米幼苗耐旱性密切相关的新的转录因子编码基因--APETALA 2（AP 2）/乙烯应答因子（ERF），ZmERF 21主要在根和叶中表达，且能被聚乙二醇诱导表达。遗传分析表明，thezmerf 21突变体表现出降低的耐旱性表型，伴随着干旱条件下常见的表型和生理变化。在干旱条件下，ZmERF 21的过表达显著提高了玉米叶绿素含量和抗氧化酶活性。RNA-Seq和DNA亲和纯化测序分析进一步揭示ZmERF 21可能通过与潜在靶基因的启动子结合，直接调控激素（乙烯、脱落酸）和Ca信号相关基因以及其他应激反应基因的表达。本研究结果为ZmERF 21参与玉米干旱胁迫反应提供了分子证据。
Drought stress adversely impacts crop development and yield. Maize frequently encounters drought stress during its life cycle. Improvement of drought tolerance is a priority of maize breeding programs. Here, we identified a novel transcription factor encoding gene,APETALA2(AP2)/Ethylene response factor(ERF), which is tightly associated with drought tolerance in maize seedlings.ZmERF21is mainly expressed in the root and leaf and it can be highly induced by polyethylene glycol treatment. Genetic analysis showed that thezmerf21mutant plants displayed a reduced drought tolerance phenotype, accompanied by phenotypical and physiological changes that are commonly observed in drought conditions. Overexpression ofZmERF21in maize significantly increased the chlorophyll content and activities of antioxidant enzymes under drought conditions. RNA‐Seq and DNA affinity purification sequencing analysis further revealed thatZmERF21may directly regulate the expression of genes related to hormone (ethylene, abscisic acid) and Ca signaling as well as other stress‐response genes through binding to the promoters of potential target genes. Our results thereby provided molecular evidence ofZmERF21is involved in the drought stress response of maize.