PRESENCE OF FIBRONECTIN DURING EARLY EMBRYOGENESIS IN AMPHIBIAN PLEURODELES-WALTLII
PRESENCE OF FIBRONECTIN DURING EARLY EMBRYOGENESIS IN AMPHIBIAN PLEURODELES-WALTLII
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DOI：
10.1016/0045-6039(83)90059-3
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
DARRIBERE, T
中科院分区：
文献类型：
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作者：
BOUCAUT, JC;DARRIBERE, T
采用间接免疫荧光和凝胶电泳方法研究了华氏原螯虾早期发育过程中纤维连接蛋白（FN）的存在。在早期囊胚和晚期原肠胚之间，细胞表面有荧光染色，特别是在囊胚腔顶和边缘区的细胞。通过原肠胚形成，细胞外物质，这是积累在该地区的囊胚孔，FN染色严重。偏高碘酸钠-硼[3 H]氢化钠用于标记表面碳水化合物残基。标记的糖蛋白，其特征在于十二烷基硫酸钠和二维聚丙烯酰胺凝胶电泳结合荧光。放射自显影显示220-K [千道尔顿]细胞表面糖蛋白，与FN单体共迁移。FN可能参与两栖动物胚胎的早期形态发生。
The presence of fibronectin (FN) was investigated during the early development of P. waltlii by using indirect immunofluorescence and gel electrophoresis methods. Between the early blastula and late gastrula stages, fluorescent staining was detected on cell surface, especially in cells from the blastocoele roof and the marginal zone. Through gastrulation, extracellular material, which was accumulated in the area of the blastopore, was heavily stained for FN. Sodium metaperiodate-sodium boro[3H]hydride was used to label the surface carbohydrate residues. Labeled glycoproteins were characterized by sodium dodecyl sulfate and 2-dimensional polyacrylamide gel electrophoresis coupled with fluorography. Autoradiograms revealed a 220-K [kilodalton] cell surface glycoprotein which was co-migrating with monomers of FN. FN may be involved in the early morphogenesis of the amphibian embryo.