Cell size and water permeability as determining factors for cell viability after freezing at different cooling rates
Cell size and water permeability as determining factors for cell viability after freezing at different cooling rates
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DOI：
10.1128/aem.70.1.268-272.2004
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发表时间：
2004-01-01
影响因子：
4.4
通讯作者：
Gervais, P
中科院分区：
文献类型：
--
作者：
Dumont, F;Marechal, PA;Gervais, P
这项工作研究了五种类型的细胞（两种酵母细胞，酿酒酵母 CBS 1171 和产朊假丝酵母；两种细菌菌株，大肠杆菌和植物乳杆菌；以及一种人类白血病 K562 细胞）的生存能力，作为冷冻过程中冷却速率的函数。研究的冷却速率范围从 5 至 30,000 摄氏度/分钟。细胞活力分为三个范围：（i）低冷却速率（5至180℃/min）的高活力，允许细胞水完全流出并且不允许任何细胞内结晶； (ii) 快速冷却速率（180 至 5,000 摄氏度/分钟）的可行性较低，这使得热流压倒水流出（在这种情况下，当水流出电池时，会发生电池水结晶）； (iii)对于非常高的冷却速率（>5,000℃/分钟）的高活力，这使得热量非常快速并在任何水从细胞流出之前诱导细胞内结晶和/或玻璃化。最后，做出了将细胞死亡与水流出细胞时细胞水结晶相关的假设。此外，这种一般细胞行为对于每种类型的细胞来说都是不同的，并且似乎受到细胞大小、透水性和细胞壁的存在的调节。
This work studied the viabilities of five types of cells (two yeast cells, Saccharomyces cerevisiae CBS 1171 and Candida utilis; two bacterial strains, Escherichia coli and Lactobacillus plantarum; and one human leukemia K562 cell) as a function of cooling rate during freezing. The range of investigated cooling rates extended from 5 to 30,000degreesC/min. Cell viability was classified into three ranges: (i) high viability for low cooling rates (5 to 180degreesC/min), which allow cell water outflow to occur completely and do not allow any intracellular crystallization; (ii) low viability for rapid cooling rates (180 to 5,000degreesC/min), which allow the heat flow to prevail over water outflow (in this case, cell water crystallization would occur as water was flowing out of the cell); (iii) high viability for very high cooling rates (>5,000degreesC/min), which allow the heat How to be very rapid and induce intracellular crystallization and/or vitrification before any water outflow from the cell. Finally, an assumption relating cell death to the cell water crystallization as water is flowing out of the cell is made. In addition, this general cell behavior is different for each type of cell and seems to be moderated by the cell size, the water permeability properties, and the presence of a cell wall.