Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5.
Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5.
复制标题
小鼠胚胎外胚层致死 tcl-w5 的 750 kb 关键区域的基因内容。
DOI：
10.1007/s00335-003-2329-1
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Nagaraja,Ramaiah
中科院分区：
文献类型：
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作者：
Abe,Kuniya;Yuzuriha,Misako;Sugimoto,Michihiko;Ko,MinoruSH;Brathwaite,Mgavi;Waeltz,Paul;Nagaraja,Ramaiah
小鼠纯合的双等位基因在胚胎外胚层发育相关缺陷的原肠胚形成中被阻止。突变基因与小鼠MHC区域的h - 2klocus（标记sh - 2pbandd17mit147）在遗传上密切相关。为了对突变基因进行定位克隆，我们构建了一个全长约1mb的BAC序列。结合公开基因组数据、EST数据库检索和基因预测程序，我们对BACs进行了定位和测序分析，将tw5关键区域的1.06 cM划分为750 kb，并推断出36个基因（1/20 kb）编码在该区间。所有测试的33个基因都被证实在胚胎组织中通过RT-PCR分析表达，在许多情况下也通过EST表达谱表达。因此，这个高度基因丰富的区域在早期发育过程中基本上完全转录，并提供了筛选胚胎病变的优先候选物。
Mice homozygous for thetw5allele arrest at gastrulation from defects associated with embryonic ectoderm development. The mutated gene has been genetically closely linked to theH-2Klocus in the mouse MHC region, flanked by markersH-2PbandD17Mit147. Aiming at the positional cloning of the mutated gene, we constructed a BAC contig spanning about 1 Mb of the genomic region. On the basis of our mapping and sequencing analysis of the BACs combined with public genome data, EST database searches, and gene prediction programs, we delimit the 1.06 cM of thetw5critical region to 750 kb, and infer 36 genes (1/20 kb) encoded in the interval. All of the 33 genes tested were confirmed as expressed in embryonic tissues by RT-PCR analyses, and in many cases by EST expression profiles as well. Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for thetw5embryonic lesion.