Fluorocarbon perfusion of the isolated rat brain: measurement of tissue spaces, EEG and oxygen uptake.
Fluorocarbon perfusion of the isolated rat brain: measurement of tissue spaces, EEG and oxygen uptake.
复制标题
离体大鼠脑的氟碳灌注：组织空间、脑电图和摄氧量的测量。
DOI：
10.1016/0304-4165(91)90220-b
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Clark,JB
中科院分区：
文献类型：
--
作者：
Harvey,SA;Trankina,ML;Olson,MS;Clark,JB
Previously, we have used the isolated perfused rat brain (IPRB) to demonstrate authentic cerebral synthesis of the lipid mediator platelet-activating-factor (Kumar, R., Harvey, S.A.K., Kester, M., Hanahan, D.J. and Olson, M.S. (1988) Biochim. Biophys. Acta 963, 375–383). The present study demonstrates that this fluorocarbon perfusion technique maintains the integrity of the blood-brain barrier (BBB), as evidenced by the small volume (1.77–3.33%) accessible to [carboxyl-14C]inulin. 51–66% of the brain was accessible to3H2O, except for the spinal cord which is poorly perfused (16% accessible to3H2O). There is no effective perfusion of muscle tissue associated with the preparation (< 6% accessible to3H2O). Fast Fourier Transform analysis of digitized EEG data showed that in low frequency bands (< 7.5 Hz) the IPRB had reduced electrical activity relative to the whole conscious animal. The GABA antagonist bicuculline, which has convulsant effects in vivo, causes a 3–4-fold increase in overall (root-mean-square) electrical activity, but decreases further the relative amplitude of low frequencies. With appropriate corrections, measurement of the oxygen consumption of the IPRB can be made without the necessity for venous cannulation. Oxygen consumption of the IPRB is flow-dependent. At a perfusion rate of 1.54 ml/min per g, unstimulated oxygen consumption of the IPRB is 2.07–2.23 μmol/min per g, or 67–72% of the consumption of the brain in vivo. Administration of bicuculline to the IPRB causes a 31% increase in lactate efflux, but only a 15% increase in oxygen uptake, suggesting that the preparation becomes functionally ischemic. Measurement of ATP/ADP levels in control and bicuculline-treated IPRBs confirms this. Other workers have used the IPRB as a model for the cerebral effects of pharmacological agents and of metabolic insult. The present study shows that under various experimental conditions oxygen uptake, analytical EEG measurements, and the integrity of the blood-brain barrier all can be monitored.
