Comparative Analysis Between Paramecium Strains with Different Syngens Using the RAPD Method
Comparative Analysis Between Paramecium Strains with Different Syngens Using the RAPD Method
复制标题
DOI：
10.1007/s00248-021-01864-y
复制
发表时间：
2021-09-15
影响因子：
3.6
通讯作者：
Watarai, Masahisa
中科院分区：
文献类型：
--
作者：
Matsumoto, Sonoko;Watanabe, Kenta;Watarai, Masahisa
草履虫是一种自由生活的原生生物，主要生活在淡水环境中。它们是具有高运动性和吞噬能力的纤毛虫，已被用于分析细胞运动性和作为病原体的宿主模型。除了这些生物学特征外，除了通常的物种形态和遗传分类外，还存在与草履虫独特繁殖相关的分类(如同源)和交配类型。在这项研究中，我们试图建立一种简单的方法来鉴定形态上难以区分的草履虫菌株，即随机扩增多态性DNA(RAPD)分析。因此，我们可以观察到菌株特有的带型。我们还证实，内共生小球藻细胞的存在影响了巴氏拟青霉的带型。此外，对几个具有不同突触基因的尾叶拟青霉菌株进行的RAPD分析结果表明，有可能检测到特定SynGen的特异条带。通过改进反应条件和随机引物，根据本研究的结果，可以将RAPD分析应用于草履虫菌株的鉴定及其SynGen确认试验。
Paramecium spp. are a genus of free-living protists that live mainly in freshwater environments. They are ciliates with high motility and phagocytosis and have been used to analyze cell motility and as a host model for pathogens. Besides such biological characteristics, apart from the usual morphological and genetic classification of species, the existence of taxonomies (such as syngens) and mating types related to Paramecium's unique reproduction is known. In this study, we attempted to develop a simple method to identify Paramecium strains, which are difficult to distinguish morphologically, using random amplified polymorphic DNA (RAPD) analysis. Consequently, we can observe strain-specific band patterns. We also confirm that the presence of endosymbiotic Chlorella cells affects the band pattern of P. bursaria. Furthermore, the results of the RAPD analysis using several P. caudatum strains with different syngens show that it is possible to detect a band specific to a certain syngen. By improving the reaction conditions and random primers, based on the results of this study, RAPD analysis can be applied to the identification of Paramecium strains and their syngen confirmation tests.