Long-term gene silencing by RNAi
Long-term gene silencing by RNAi
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DOI：
10.1038/442882a
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发表时间：
2006-08-24
期刊：
影响因子：
64.8
通讯作者：
Plasterk, Ronald H. A.
中科院分区：
文献类型：
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作者：
Vastenhouw, Nadine L.;Brunschwig, Karin;Plasterk, Ronald H. A.
小RNA分子参与细胞中的各种活动：在称为RNA干扰（RNAi）的过程中，双链RNA触发具有匹配序列的信使RNA的降解;该过程的小RNA中间体也可以修饰细胞核中的基因表达。在这里，我们表明，一个单一的事件的RNAi在线虫cabelegan可诱导转录沉默的影响，是无限期地遗传在没有原来的触发。我们的研究结果可能有助于正在进行的RNAi治疗疾病的发展。
Small RNA molecules participate in a variety of activities in the cell: in a process known as RNA interference (RNAi), double-stranded RNA triggers the degradation of messenger RNA that has a matching sequence; the small RNA intermediates of this process can also modify gene expression in the nucleus. Here we show that a single episode of RNAi in the nematodeCaenorhabditis eleganscan induce transcriptional silencing effects that are inherited indefinitely in the absence of the original trigger. Our findings may prove useful in the ongoing development of RNAi to treat disease.