MRI guidance improves accuracy of stereotaxic targeting for cell transplantation in parkinsonian monkeys
MRI guidance improves accuracy of stereotaxic targeting for cell transplantation in parkinsonian monkeys
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DOI：
10.1016/j.expneurol.2004.11.032
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发表时间：
2005-05-01
影响因子：
5.3
通讯作者：
Bakay, R
中科院分区：
文献类型：
--
作者：
Subramanian, T;Deogaonkar, M;Bakay, R
靶向的准确性是中枢神经系统细胞移植成功的关键。我们比较了常规阿特拉斯引导的立体定向与磁共振成像（MRI）引导的立体定向靶向在帕金森猴基底节区各种核中的准确性。28只猴子接受单侧纹状体移植。在15只猴子的大脑中使用了高分辨率的3D MR图像，这些图像安装了与mri兼容的立体定位框架，用于目标定位。这是紧随其后的颅骨手术与框架“原位”。另外13只猴子接受了立体定向阿特拉斯引导的颅骨手术，以放置细胞移植。经过广泛的行为测试和微电极记录，所有动物都被灌注。脑冠状切片和染色，以确定针束的形态作为立体定位位置的精确测量。mri引导下的立体定向80%完全准确，而阿特拉斯引导下的立体定向为38.5%。在阿特拉斯引导下，完全丢失目标的几率高达38.5%，而在MRI引导下，完全丢失目标的几率降至6.67%。定位错误主要发生在前尾状核和后壳核，而前壳核的准确性较好。这些结果表明，高分辨率的3D磁共振成像可以提高猴子单侧颅骨立体定向瞄准壳核和尾状核的准确性。(c) 2004 Elsevier Inc.版权所有。
Accuracy of targeting is critical for the success of cell transplantation in the central nervous system. We compared the accuracy of conventional atlas-guided stereotaxis to magnetic resonance imaging (MRI)-guided stereotaxic targeting in various basal ganglia nuclei in parkinsonian monkeys. 28 monkeys underwent unilateral striatal transplantation. High-resolution 3D MR images of the brain were used in 15 monkeys fitted with a MRI-compatible stereotaxic frame for target localization. This was immediately followed by cranial surgery with the frame "in situ". 13 additional monkeys underwent stereotaxic atlas-guided cranial surgery for placement of cell transplants. Following extensive behavioral testing and microelectrode recordings, all animals were perfused. The brains were sectioned coronally and stained to determine the morphology of needle tracts as an accuracy measure of stereotaxic placements. MRI-guided stereotaxy was completely accurate in 80% as compared to 38.5% in atlas-guided stereotaxis. The chance of missing a target completely was as high as 38.5% in atlas-guided stereotaxis, which was reduced to 6.67% when MRI was used for guidance. Targeting error occurred mostly in the anterior caudate and posterior putamen as against better accuracy in the anterior putamen. These results suggest that accuracy of stereotaxic unilateral cranial targeting into the putamen and the caudate in monkeys can be improved with high-resolution 3D MR imaging. (c) 2004 Elsevier Inc. All rights reserved.