A SPECIFIC DEFECT IN CD3 GAMMA-CHAIN GENE-TRANSCRIPTION RESULTS IN LOSS OF T-CELL RECEPTOR CD3 EXPRESSION LATE AFTER HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION OF A CD4+ T-CELL LINE
A SPECIFIC DEFECT IN CD3 GAMMA-CHAIN GENE-TRANSCRIPTION RESULTS IN LOSS OF T-CELL RECEPTOR CD3 EXPRESSION LATE AFTER HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION OF A CD4+ T-CELL LINE
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DOI：
10.1073/pnas.87.17.6713
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发表时间：
1990-09-01
影响因子：
11.1
通讯作者：
KETTMANN, R
中科院分区：
文献类型：
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作者：
WILLARDGALLO, KE;VANDEKEERE, F;KETTMANN, R
研究了体外人免疫缺陷病毒（1型）感染CD 4 + T细胞系后对细胞蛋白表达的顺序影响。人白细胞介素2依赖性辅助性T细胞系WE 17/10中的事件在几个方面与获得性免疫缺陷综合征的临床进展相似。WE 17/10细胞感染的特征在于病毒复制发生的延长期，在此期间不伴随细胞毒性，并且表面CD 4最多减少30%。在感染的第一阶段，细胞蛋白表达通常不受影响。然而，2-3个月后，细胞表面和细胞内部的T细胞受体/CD 3复合物表达的严重缺陷变得明显。其他细胞膜标志物，如CD 2和CD 25，在整个感染过程中保持不变;在其初始下降后，CD 4保持在对照值的70%。TCR/CD 3复合物的表面表达的缺乏与CD 3 γ-受体的转录中的特异性缺陷相关。链基因
Sequential effects on cellular protein expression following human immunodeficiency virus (type 1) infection of a CD4+ T-cell line in vitro were investigated. Events in the human interleukin 2-dependent helper T-cell line WE17/10 are similar in several respects to the clinical progression in acquired immunodeficiency syndrome. WE17/10 cell infection is characterized by an extended period during which viral replication occurs without accompanying cytotoxicity and with a maximum 30% decrease in surface CD4. Cellular protein expression generally remains unaffected during this first phase of infection. However, after 2-3 months, a severe defect in the expression of the T-cell receptor/CD3 complex both on the cell surface and inside the cell becomes apparent. Other cell membrane markers, such as CD2 and CD25, remain constant throughout the course of infection; after its initial decrease, CD4 remains at 70% of control values. Lack of surface expression of the TCR/CD3 complex is correlated with a specific defect in transcription of the CD3 .gamma.-chain gene.