Regulation of trehalase expression inhibits apoptosis in diapause cysts of Artemia.
Regulation of trehalase expression inhibits apoptosis in diapause cysts of Artemia.
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DOI：
10.1042/bj20131020
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发表时间：
2013-12
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影响因子：
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通讯作者：
Fan Yang;Su Chen;Zhong-Min Dai;Dian-fu Chen;Ru-Bing Duan;Hong-liang Wang;Sheng-Nan Jia;Wei-Jun Yang
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文献类型：
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作者：
Fan Yang;Su Chen;Zhong-Min Dai;Dian-fu Chen;Ru-Bing Duan;Hong-liang Wang;Sheng-Nan Jia;Wei-Jun Yang
海藻糖酶在海藻糖的代谢中起着重要的作用，它能将海藻糖特异性地分解成葡萄糖。在卤虫的滞育包囊胚胎(囊)中储存了大量的海藻糖，这不仅对卤虫的抗逆性至关重要，而且为滞育终止后的发育提供了能量来源。在本研究中，我们描述了孤雌卤虫海藻糖酶的转录调控机制。在卤虫滞育包囊中发现了一种海藻糖酶相关蛋白(ArTAP)。ArTAP仅在滞育胚胎中表达。进一步的分析表明，ArTAP可以与海藻糖酶基因的特定内含子片段结合。RNAi敲除ArTAP后，包囊释放，包囊内有两个甲壳素结合蛋白缺失。Western blotting显示，与对照组相比，ArTAP基因敲除的包囊中海藻糖酶水平升高，并诱导细胞凋亡。综上所述，这些结果表明，ArTAP是海藻糖酶表达的关键调节者，而海藻糖酶在滞育孢子形成过程中对海藻糖代谢起着重要作用。
Trehalase, which specifically hydrolyses trehalose into glucose, plays an important role in the metabolism of trehalose. Large amounts of trehalose are stored in the diapause encysted embryos (cysts) of Artemia, which are not only vital to their extraordinary stress resistance, but also provide a source of energy for development after diapause is terminated. In the present study, a mechanism for the transcriptional regulation of trehalase was described in Artemia parthenogenetica. A trehalase-associated protein (ArTAP) was identified in Artemia-producing diapause cysts. ArTAP was found to be expressed only in diapause-destined embryos. Further analyses revealed that ArTAP can bind to a specific intronic segment of a trehalase gene. Knockdown of ArTAP by RNAi resulted in the release of cysts with coarse shells in which two chitin-binding proteins were missing. Western blotting showed that the level of trehalase was increased and apoptosis was induced in these ArTAP-knockdown cysts compared with controls. Taken together, these results show that ArTAP is a key regulator of trehalase expression which, in turn, plays an important role in trehalose metabolism during the formation of diapause cysts.