THE EPIDEMIOLOGY OF MULTIPLE-CLONE PLASMODIUM-FALCIPARUM INFECTIONS IN GAMBIAN PATIENTS
THE EPIDEMIOLOGY OF MULTIPLE-CLONE PLASMODIUM-FALCIPARUM INFECTIONS IN GAMBIAN PATIENTS
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DOI：
10.1017/s0031182000059217
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发表时间：
1991-08-01
期刊：
影响因子：
2.4
通讯作者：
MCBRIDE, JS
中科院分区：
文献类型：
--
作者：
CONWAY, DJ;GREENWOOD, BM;MCBRIDE, JS
多克隆恶性疟原虫单倍体血液期感染的发生是在蚊子二倍体阶段进行异受精和遗传交换的先决条件。使用单克隆抗体对3多态性血液阶段抗原，双色差分免疫荧光的方法允许之间的分辨率1和4个克隆/分离。在冈比亚的疟疾患者血液中平均检测到2个恶性疟原虫克隆。克隆/患者的平均数与年龄、寄生虫血症或疾病严重程度无关。有一个样本期间之间的平均数略有差异，可能反映了传输强度的时间差异。2位点的遗传多样性的克隆内分离的统计分析得出的结论是，不是所有的多克隆感染的重复感染的结果，但有些是由于单一的多克隆接种。
The occurrence of multiple-clone Plasmodium falciparum haploid blood-stage infections is a pre-requisite for cross-fertilization and genetic exchange at the diploid stage in the mosquito. Using monoclonal antibodies against 3 polymorphic blood-stage antigens, a method of two-colour differential immunofluorescence allowed the resolution of between 1 and 4 clones/isolate. A mean of 2 P falciparum clones was detected in the blood of malaria patients in The Gambia. The mean number of clones/patient showed no correlation with age, parasitaemia, or disease severity. There was a slight difference in mean number between sample periods, probably reflecting temporal differences in transmission intensity. A statistical analysis of 2-locus genetic diversity of clones within isolates concludes that not all multiple-clone infections result from superinfection, but that some are due to single multiple-clone inoculations.