Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes:: Infrared spectroscopic characterization at the air/water interface
Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes:: Infrared spectroscopic characterization at the air/water interface
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DOI：
10.1021/jp062537q
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发表时间：
2006-11-09
影响因子：
3.3
通讯作者：
Losche, Mathias
中科院分区：
文献类型：
--
作者：
Dyck, Martina;Kerth, Andreas;Losche, Mathias
本文用外红外反射吸收光谱法研究了神经肽Y（NPY）在空气/水界面及与作为亚相的水表面磷脂单分子膜的缔合作用。NPY的构象和取向的研究表明，它采用α-螺旋结构，并在纯肽单层中平行于空气/水界面取向。二级结构和方向都保留在混合脂质/NPY单层。与两性离子DPPC和阴离子DMPS相关的NPY的比较表明，静电吸引对肽与膜表面的结合起主要作用。
The association of neuropeptide Y (NPY) at the air/water interface and with phospholipid monolayers on water as subphase has been investigated using external infrared reflection absorption spectroscopy (IRRAS). Studies of the conformation and orientation of NPY suggest that it adopts an alpha-helical structure and is oriented parallel to the air/water interface in neat peptide monolayers. Both secondary structure and orientation are preserved in mixed lipid/NPY monolayers. Comparison of NPY associated with zwitterionic DPPC and with anionic DMPS suggests that electrostatic attraction plays a major role for peptide binding to the membrane surface.