MHC class I A loci polymorphism and diversity in three Southeast Asian populations of cynomolgus macaque
MHC class I A loci polymorphism and diversity in three Southeast Asian populations of cynomolgus macaque
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DOI：
10.1007/s00251-009-0390-y
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发表时间：
2009-09-01
期刊：
影响因子：
3.2
通讯作者：
Shiina, Takashi
中科院分区：
文献类型：
--
作者：
Kita, Yuki F.;Hosomichi, Kazuyoshi;Shiina, Takashi
食蟹猴（Macaca fascicularis，Mafa）已成为生物医学研究的重要动物模型，需要对其主要组织相容性复合体多态性区域进行更广泛的表征。与更常研究的恒河猴Mafa I A类基因座相比，目前关于多基因Mafa I A类基因座的多态性或多样性的信息是有限的。因此，在本文中，为了更好地阐明Mafa-A1的多态性和遗传差异的程度和类型在三个本地东南亚人群（印度尼西亚，越南和菲律宾），并探讨如何猕猴和人类之间的等位基因差异可能已经演变到影响各自的免疫反应，我们确定了83 Mafa-A基因座衍生的等位基因的DNA测序，其中66个是新描述的。大多数等位基因是独特的，每个人口，但七个最常见的等位基因序列相同的一些等位基因在其他猕猴物种。通过分析群体等位基因频率、Hardy-Weinberg平衡、杂合度、核苷酸多样性谱和遗传多样性，揭示了群体间等位基因变异的巨大和动态的遗传和结构差异和相似性;通过Wright的FST统计和分子方差的层次分析揭示了群体间遗传结构的差异，（3）通过Tajima's D中立性检验，分析了三个群体所受的不同的人口压力和选择压力。在Mafa-A1的大水平的多样性和多态性是不太明显，在菲律宾比在越南或印度尼西亚的人口，这可能有重要的意义，在动物捕获，选择和育种的医学研究。
Cynomolgus macaques (Macaca fascicularis, Mafa) have emerged as important animal models for biomedical research, necessitating a more extensive characterization of their major histocompatibility complex polymorphic regions. The current information on the polymorphism or diversity of the polygenetic Mafa class I A loci is limited in comparison to the more commonly studied rhesus macaque Mafa class I A loci. Therefore, in this paper, to better elucidate the degree and types of polymorphisms and genetic differences of Mafa-A1 among three native Southeast Asian populations (Indonesian, Vietnamese, and Filipino) and to investigate how the allele differences between macaques and humans might have evolved to affect their respective immune responses, we identified 83 Mafa-A loci-derived alleles by DNA sequencing of which 66 are newly described. Most alleles are unique to each population, but seven of the most frequent alleles were identical in sequence to some alleles in other macaque species. We also revealed (1) the large and dynamic genetic and structural differences and similarities in allelic variation by analyzing the population allele frequencies, Hardy-Weinberg's equilibrium, heterozygosity, nucleotide diversity profiles, and phylogeny, (2) the difference in genetic structure of populations by Wright's FST statistic and hierarchical analysis of molecular variance, and (3) the different demographic and selection pressures on the three populations by performing Tajima's D test of neutrality. The large level of diversity and polymorphism at the Mafa-A1 was less evident in the Filipino than in the Vietnam or the Indonesian populations, which may have important implications in animal capture, selection, and breeding for medical research.